荆半夏遗传多样性的SRAP分析

利用SRAP技术对10种不同叶形荆半夏的遗传多样性及亲缘关系进行了分析。40对SRAP引物中有39对表现多态性，共获得632条多态性带，平均每个引物产生16.2个多态性条带。根据SRAP标记的结果，采用UPGMA法进行聚类分析，遗传相似系数为0.105～0.978，将10种不同叶形荆半夏划分为5类：聚类结果很明显地将三叶荆半夏和五叶荆半夏区分为两大类；三叶半夏大类中又可分为4个亚类：Ⅰ类：无缺刻的耳叶形荆半夏、尖叶形荆半夏、圆叶形荆半夏、叠叶形荆半夏、窄芍药形荆半夏和芍药形荆半夏；Ⅱ类：耳叶形荆半夏（缺刻）；Ⅲ类：宽狭叶形荆半夏；Ⅳ类：狭叶形荆半夏。该研究为半夏属植物的鉴定和遗传研究提供了新的DNA分子标记技术手段，并从分子水平上揭示荆半夏的遗传背景、亲缘关系。

Genetic Diversity Analysis of Pinellia ternata in Jingzhou by SRAP Makers

The genetic diversity and relationship of 10 P.ternta of JingZhou with different leaf shape were analyzed by SRAP. The DNAS of 10 P.ternta of JingZhou were tested with SRAP using 40 primer pairs and 39 primer pairs showed polymorphism. A total of 632 polymorphic bands were obtained and there were 16.2 polymorphic bands of per primer pairs. The SRAP based genetic similarity ranged 0.105~0.978, UPGMA cluster analysis based on SRAP molecular markers data divided 10 different leaf shape into five groups: the result of UPGMA divided trifoliate P.ternta of JingZhou and quinquefoliolate P.ternta of JingZhou into two group. There are four small group in trifoliate P.ternta of JingZhou:Ⅰ: auricular leaf、cuspate leaf、rounded leaf、terrace leaf、thin peony leaf and peony leaf；Ⅱ: auricular leaf with emargination；Ⅲ: wide angustifoliate；Ⅳ: angustifoliate. This study provided an new marker system for P.ternta variety identification and genetic research, which would be helped to reveal the genetic background and relationship of P.ternta germplasms .