| 华山松SRAP引物的筛选与验证 |
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| 引用本文： | 叶鹏, 瞿绍宏, 李云芳, 等. 华山松SRAP引物的筛选与验证[J]. 西南林业大学学报（自然科学）, 2020, 40(1): 22–27.doi:10.11929/j.swfu.201904012 |
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| 作者姓名： | 叶鹏 瞿绍宏 李云芳 罗正平 董云祥 刘成 董章宏 鲁华 辛培尧 |
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| 作者单位： | 1. 西南林业大学西南山地森林资源保育与利用教育部重点实验室，云南 昆明 650233;2. 云龙县漕涧林场，云南 大理 672711;3. 楚雄市紫溪山省级自然保护区管理局，云南 楚雄 675000 |
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| 摘 要： | 为筛选华山松SRAP遗传多样性引物，以华山松无性系种子园6个种源的30株优良无性系幼嫩无病害针叶为实验材料，基于SRAP标记技术，选用10条正向引物、10条反向引物，随机组合成100个SRAP引物组合，对华山松DNA进行PCR扩增。结果表明：实验共筛选获得15对多态性较为丰富并且具有可重复性的有效引物；15对引物共扩增出3 767条DNA条带，其中，多态性条带有2 448条，占总条带数的64.99%，平均多态性比率为64.28%。所得15对SRAP引物适合作为华山松遗传多样性分析。
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| 关 键 词： | 华山松 SRAP 引物筛选 验证 |
| 收稿时间： | 2019-04-03 |
Selection and Validation of SRAP Primers in Pinus armandii |
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| Peng Ye, Shaohong Qu, Yunfang Li, Zhengping Luo, Yunxiang Dong, Cheng Liu, Zhanghong Dong, Hua Lu and Peiyao Xin. Selection and Validation of SRAP Primers in Pinus armandii[J]. Journal of Southwest Forestry University, 2020, 40(1): 22-27.doi:10.11929/j.swfu.201904012 |
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| Authors: | Peng Ye Shaohong Qu Yunfang Li Zhengping Luo Yunxiang Dong Cheng Liu Zhanghong Dong Hua Lu Peiyao Xin |
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| Affiliation: | 1. Key Laboratory of Ministry of Education for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Southwest Forestry University, Kunming Yunnan 650233, China;2. Caojian Forest Farm of Yunlong County, Dali Yunnan 672711, China;3. Administration Bureau of Zixishan Provincial Nature Reserve, Chuxiong Yunnan 675000, China |
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| Abstract: | In order to screen primers for SRAP genetic diversity of Pinus armandii, young disease-free needles from 30 superior clones from 6 provenances of P. armandii clonal seed orchard were used as experimental materials. Based on SRAP marker technique, P. armandii DNA were amplified with 100 SRAP primers combinations combined randomly with 10 forward and 10 reverse primers. It was found that a total of 15 pairs of effective primers with rich polymorphism and high repeatability were screened. A total of 3 767 DNA bands were amplified by 15 pairs of primers. Among them, 2 448 polymorphic bands accounted for 64.99% of the total bands, with an average polymorphic rate of 64.28%. The 15 pairs of SRAP primers obtained from the experiment were suitable for the genetic diversity analysis of P. armandii. |
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| Keywords: | Pinus armandii SRAP primer selection validation |
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