中国湖桑地方品种遗传多样性分析

[目的]探讨中国湖桑地方品种的遗传多样性。[方法]采用ISSR分子标记技术,对141个湖桑品种的遗传多样性进行分析,并根据ISSR标记遗传相似系数,按UPGMA法对141份湖桑地方品种进行聚类分析。[结果]共扩增出90个条带,其中多态性条带57条,多态性比率63.33%。141份湖桑的遗传相似系数变异范围为0.633 3~1.000 0,平均遗传相似系数为0.483 4,表明不同湖桑品种间的遗传多样性存在差异。但聚类分析结果与传统的形态学和农艺学性状的分类结果不完全一致。[结论]4个亚类清楚地代表了141份湖桑的遗传关系,并为桑树品种的优化和种质资源的保护提供了理论依据。

Genetic Diversity of Chinese Hu mulberry (Morus L.)Based on ISSR Analysis

[Objective]To investigate the genetic diversity of local varieties of Chinese Hu mulberry(Morus L.).[Method]The genetic diversity of 141 copies of Hu mulberry varieties was analyzed by ISSR molecular markers.A dendrogram of all 141 Hu mulberry varieties based on the genetic similarity coefficients using ISSR molecular markers was generated by UPGMA cluster method.[Result] 12 ISSR primers had amplified a tota1 of 90 amplified,of which 57 bands were polymorphic,and the polymorphic rate was 63.33%.The genetic similarity coefficients of 141 Hu mulberry germplasm resources varied from 0.633 3 to 1.000 0 with the average of 0.483 4,indicating that there was difference on genetic diversity among different varieties of Hu mulberries.Clustering of the 141 Hu mulberry varieties did not correspond with the conventional morphology and agronomy characteristics.[Conclusion]Four subgroups clearly represent the genetic relationships in the 141 accessions which are benefit for the variety improvement and germplasm resource conservation.