高纯度银杏黄酮的制备

[目的]制备高纯度的银杏黄酮,探索一条规模化制备高纯度银杏总黄酮的工艺路线。[方法]用固相填料为ODS的色谱柱纯化银杏黄酮,考察梯度洗脱、浓缩温度、干燥温度对银杏黄酮含量的影响。[结果]甲醇∶0.5%磷酸=5∶5和7∶3（V∶V）的馏分为目标馏分,最佳浓缩温度和干燥温度为45℃,在最佳条件下,银杏黄酮质量百分含量≥90%,收率为78.3%。[结论]该试验获得了高纯度银杏黄酮,为高纯度的银杏总黄酮制备提供了一种高效的方法。

Preparation of High-Purity Flavonoids from Ginkgo biloba L.

[Objective] To prepare highly purified flavonoids of Ginkgo biloba L.,so as to explore a large-scale preparation process line of high purity flavonoids from Ginkgo biloba L..[Method] Purified with chromatographic column with packing of ODS,effects of mobile phase ratio,concentration temperature and drying temperature on yield of flavonoids from Ginkgo biloba L.were investigated.[Result] The optimum preparation conditions were as follows: target fraction of methanol to 0.5% phosphoric acid ratio of 5∶5 and 7∶3,concentration temperature and drying temperature of 45 ℃.The content of flavonoids from Ginkgo biloba L.was more than 90%,and the yield reached 78.3% under the optimal preparation process.[Conclusion] High-purity flavonoids from Ginkgo biloba L.were obtained,which provided an effective method for the preparation of highly purified Ginkgo biloba L.flavonoids.