| 甘蓝型油菜BnPex16p基因cDNA的克隆与表达 |
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| 引用本文： | 吴学龙,刘智宏,何海燕,郎春秀,陈锦清.甘蓝型油菜BnPex16p基因cDNA的克隆与表达[J].中国油料作物学报,2011,33(3):203-209. |
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| 作者姓名： | 吴学龙 刘智宏 何海燕 郎春秀 陈锦清 |
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| 作者单位： | 浙江省植物基因工程代谢重点实验室，浙江省农业科学院病毒学与生物技术研究所，浙江 杭州 310021
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| 基金项目： | 国家863计划，浙江省自然科学基金 |
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| 摘 要： | 利用模式植物拟南芥AtPex16p/sse1基因序列与油菜数据库BBSRC BrassicaDB比对，得到甘蓝型油菜(Brassica napus)同源EST序列，拼接出油菜Pex16p基因全长cDNA电子克隆，然后设计全长引物，以甘蓝型油菜种子cDNA为模板，克隆Pex16p基因，得到全长为1 101bp的cDNA，编码366个氨基酸的蛋白，命名为BnPex16p。BnPex16p基因序列与拟南芥AtPex16p/sse1同源，其蛋白与拟南芥同源蛋白具有完全相同的PTS2型过氧化物酶体定位肽，进化关系相近。半定量PCR(RT-PCR)发现BnPex16p基因在油菜幼嫩的根、茎、叶和种子中均有高丰度表达，在种子发育过程的中期和后期表达水平增加，暗示该基因在油脂的积累中有重要作用。
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| 关 键 词： | 过氧化物酶体 油菜Pex16p基因 序列分析 甘蓝型油菜 |
Molecular cloning and characterization of BnPex16p gene from Brassica napus |
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| WU Xue-long,LIU Zhi-hong,HE Hai-yan,LANG Chun-xiu,Chen Jin-qing.Molecular cloning and characterization of BnPex16p gene from Brassica napus[J].Chinese Journal of Oil Crop Sciences,2011,33(3):203-209. |
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| Authors: | WU Xue-long LIU Zhi-hong HE Hai-yan LANG Chun-xiu Chen Jin-qing |
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| Institution: | WU Xue-long,LIU Zhi-hong,HE Hai-yan,LANG Chun-xiu,Chen Jin-qing(Zhejiang Provincial Key Laboratory of Plant Metabolic Engineering,Institute of Virology and Biotechnology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China) |
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| Abstract: | According to AtPex16p/SSE1 and ESTs sequences from Brassica napus by wu-blast searching in BBSRC BrassicaDBA,a full-length cDNA encoding Pex16p from developing seeds of Brassica napus var CY2 was cloned.The gene was designated as BnPex16p which was 1 101bp in length encoding 366 amino acids.It was found that BnPex16p gene was homologous to AtPex16p/SSE1.They both had a PTS2 type peroxisome targeting signal and a close evolutionary distance.Semi-quantitative RT-PCR analysis demonstrated that BnPex16p was con... |
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| Keywords: | Peroxisome BnPex16p Sequence analysis Brassica napus |
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