一氧化氮在脱落酸和黑暗诱导蚕豆气孔关闭中的作用

目的]研究一氧化氮（NO）在脱落酸（ABA）和黑暗诱导的蚕豆（Vicia faba）气孔关闭中的作用。方法]以蚕豆叶片下表皮为材料,借助表皮条分析和激光扫描共聚焦显微镜技术对NO在ABA和黑暗诱导的气孔关闭中的作用进行了探索。结果]ABA和黑暗都能诱导蚕豆气孔关闭,而且ABA和黑暗诱导都能提高保卫细胞胞质内的NO水平。NO专一性清除剂2,4-羧基苯-4,4,5,5-四甲基咪唑-1-氧-3-氧化物（cPTIO）、一氧化氮合酶（NOS）抑制剂NG-氮-L-精氨酸-甲酯（L-NAME）能够大大抵消ABA和黑暗诱导气孔关闭的效应,并能阻断ABA和黑暗诱导的气孔保卫细胞内NO水平的提高,表明NO是ABA和黑暗诱导蚕豆气孔关闭的共同信号分子。结论]该研究可为探索保卫细胞信号转导网络积累一定的资料,并为提高植物抗逆能力和促进农业生产提供理论依据。

Effects of Nitric Oxide in ABA- and Darkness-Induced Stomatal Closure of V. faba

Objective] The role of NO in ABA-and darkness-induced stomatal closure in V.faba was studied.Method] The abaxial surface of V.faba leaf was selected as the material,the research was carried out by epidermal strip bioassay and laser-scanning confocal microscopy.Result] The results showed that both phytohormones ABA and darkness induced stomatal closure in V.faba.Furthermore,ABA and darkness could also improve the NO level of stomatal guard cells.ABA-and darkness-induced stomatal closure was largely prevented by 2,4-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide(cPTIO),a specific NO scavenger and NG-nitro-L-arg-methgl eater(L-NAME),an inhibitor of NO synthase(NOS) in mammalian cells that also inhibit plant NOS.The data indicate that NO is the common signaling molecule in ABA-and darkness-induced stomatal movement in V.faba.Conclusion] The study can accumulate information for seeking network of the guard cell signal conduction pathway,and provide theoretical support for improving plant resistance and promoting agricultural production.