| 新生仔猪小肠上皮细胞体外培养的研究 |
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| 引用本文： | 李莉,高淑静,张守全,陈俊伟,陆咸赏,罗园,江青艳. 新生仔猪小肠上皮细胞体外培养的研究[J]. 仲恺农业技术学院学报, 2010, 23(1) |
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| 作者姓名： | 李莉 高淑静 张守全 陈俊伟 陆咸赏 罗园 江青艳 |
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| 作者单位： | 华南农业大学,动物科学学院,广东,广州,510642 |
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| 基金项目： | 国家自然科学基金委-广东联合基金 |
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| 摘 要： | 采用灌肠消化、组织块消化、机械刮取+胰蛋白酶消化和机械刮取+胶原酶消化等4种方法对仔猪小肠黏膜上皮细胞(Intestinal epithelial cell, IEC)的体外分离培养进行了比较.结果表明,使用机械刮取+胶原酶消化法分离IEC,可简便快速地获得数量大、活性高的小肠上皮细胞,其中原代培养细胞24 h出现贴壁,7～8 d明显增殖,10～12 d贴壁细胞汇合成片,18～21 d细胞呈"铺路石"状;传代培养细胞3 h出现贴壁,3～4 d快速增殖.采用碱性磷酸酶显色反应检测,90%以上的贴壁细胞呈阳性反应.
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| 关 键 词： | 猪 小肠 上皮细胞 原代培养 传代培养 |
Studies on isolation and primary culture of neonate piglet intestinal epithelial cell |
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| LI li,GAO Shu-jing,ZHANG Shou-quan,CHEN Jun-wei,LU Xian-shang,LUO Yuan,JIANG Qing-yan. Studies on isolation and primary culture of neonate piglet intestinal epithelial cell[J]. Journal of Zhongkai Agrotechnical College, 2010, 23(1) |
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| Authors: | LI li GAO Shu-jing ZHANG Shou-quan CHEN Jun-wei LU Xian-shang LUO Yuan JIANG Qing-yan |
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| Affiliation: | LI li,GAO Shu-jing,ZHANG Shou-quan,CHEN Jun-wei,LU Xian-shang,LUO Yuan,JIANG Qing-yan(College of Animal Sciences,South China Agricultural University,Guangzhou 510642,China) |
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| Abstract: | In order to obtain pure,viable,and functional intestinal epithelial cells(IEC) from piglets,four dissociation methods including coloclysis digestion,organization block digestion,mechanical scrape plus trypsin digestionand and mechanical scrape plus collagenase digestion were tested.The results showed that mechanical scrape plus collagenase digestion was the best method to isolate IEC.About 24 hours after inoculation,IEC attached to the surface of the container.The cells proliferated obviously at 7~8 days of... |
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| Keywords: | pig small intestine epithelial cell primary culture passage culture |
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