High-performance liquid chromatographic analysis of anthocyanins in Japanese garden iris and its wild forms |
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Authors: | Tsutomu Yabuya |
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Affiliation: | (1) Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell University, Geneva, NY, USA |
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Abstract: | Summary Following bud pollination of tartary buckwheat (Fagopyrum tataricum) with pollens from common buckwheat (F. esculentum), cv. Mancan, 31% of the ovaries began to grow but all turned brown and withered after 6 to 14 days. Fluorescence microscopy of the growing ovaries showed the pollen-tube entering the embryo-sac. The ovules from the growing ovaries failed to produce any embryo in the culture medium in which the immature embryos from the self-pollinated (compatible) tartary flowers were able to mature and germinate. No embryo development was observed after cross-pollination. The interspecific incompatibility is attributed to the failure of gametes to fuse.Abbreviations BAP benzylaminopurine - IAA indoleacetic acid |
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Keywords: | Fagopyrum tataricum Fagopyrum esculentum buckwheat interspecific hybridization ovule culture |
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