不同双糖组合对猪精子冷冻保存效果的影响

为了评价不同的双糖（乳糖、蔗糖和海藻糖）添加于卵黄基础稀释液中对猪精子的冷冻保护作用，实验采用了7个冷冻保护液配方，其中第一个配方为前期实验筛选得到的乳糖卵黄稀释液（LEY），将蔗糖和海藻糖以不同的比例完全或部分替代第一个配方中的乳糖成分而获得另6个配方。精子冻后质量评价活力、顶体完整率、低渗肿胀率及畸形率等指标。结果表明，当稀释液中只添加乳糖时（LEY液，A组），猪精子的冻后活力最低；当用蔗糖或海藻糖部分替代或完全替代乳糖时，猪精子冻后各项指标都有所改善。当33.3%的乳糖和66.7%的蔗糖进行组合（F组）时，猪精子冻后活力达37.0%±2.6%，显著优于其他各组（P＜0.05），其他各项指标也显著高于单一的乳糖组（A组）。研究结果表明，将不同双糖以不同比例组合添加于猪精液冷冻保护液后，以33.3%的乳糖和66.7%的蔗糖的组合（F组）对猪精液冷冻保护的效果最好。

The Cryoprotective Effects of Different Dioses Combination on Boar Spermatozoa Quality

To evaluate the effects of different doses (lactose, sucrose and trehalose) and its combining supplementation on boar spermatozoa cryopreserved in an egg yolk based extender, seven different extenders were used to freezing semen samples. The first extender, lactose egg yolk extender (LEY), was from our previous study. Sucrose and trehalose were added into the LEY diluents， completely or partly instead of lactose，to create another six extenders. Sperm qualities were assessed by motility, acrosome integrity and hypoosmotic swelling test and sperm abnormality rate response upon immediately collection, after freezing and thawing. The results showed that, as the basis of dilution of cryopreservation, LEY showed the poorest motility among the seven extenders. When sucrose and trehalose were added to the LEY diluents instead of lactose completely or partly, the quality of frozen-thawed sperm were improved. The frozen-thawed sperm motility was significantly higher(37.0%±2.6%) than the other six extenders when 33.3% lactose and 66.7% sucrose combined group (F) (P＜0.05), the acrosome integrity and hypoosmotic swelling test were higher than LEY. In conclusion， when sucrose and trehalose were added to the LEY diluents instead of lactose completely or partly, a better boar cryopreservation semen quality appeared. And the volume fraction of 33.3% lactose and 66.7% sucrose group (F) was the best.