利用DH和IF2群体检测甘蓝型油菜株高相关性状QTL

株高是油菜重要的农艺性状之一。以油菜品种Express、SWU07构建的包含261个株系的DH群体和由其构建的包含234个株系的IF₂群体为材料, 分析2年环境下株高及其相关性状QTL表明, 在2个群体的各年份环境中总共检测到41个株高及其相关性状QTL, 分布于甘蓝型油菜的13条染色体上, 其中9个与株高相关的QTL, 分布于A02、A09、C01、C02和C06连锁群, 分别揭示了3.85%~13.34%的表型变异, 15个与主花序长度相关的QTL, 分布于A01、A02、A05、A08、A09、C01、C03和C05连锁群, 分别揭示了3.82%~9.52%的表型变异; 11个与第1分枝高度相关的QTL, 分布于A01、A03、A09、C01和C03连锁群, 分别揭示了4.01%~16.54%的表型变异; 4个与分枝区段长相关的QTL, 分布于甘蓝型油菜的A07、A09、C03和C04连锁群, 揭示了4.79%~8.10%的表型变异; 2个与平均节间长相关的QTL, 分布于A07和C05连锁群, 分别揭示了4.29%~6.04%的表型变异。其中5个QTL在不同年份环境或不同群体中被重复检测到。这些QTL为油菜株高的遗传改良提供了有用的信息。

Detection of QTLs for Plant Height Related Traits in Brassica napus L. Using DH and Immortalized F2 Population

Plant height is one of the important agronomic traits in rapeseed. In this study, QTLs for plant height and plant height related traits in Brassica napus were evaluated in two years environments by using a doubled haploid (DH) population lines derived from the cross between two rapeseed cultivars, Express and SWU07, and an immortalized F₂ population generated by randomly permuted intermating of these DH lines. A total of 41 putative QTLs were identified for plant height and plant height related traits and located on 13 linkage groups. Among them, nine QTLs were identified for plant height, located on A02, A09, C01, C02, and C06, respectively, with explained phenotypic variation ranging from 3.85% to 13.34%, 15 QTLs were identified for inflorescence length, located on A01, A02, A05, A08, A09, C01, C03, and C05, respectively, with explained phenotypic variation ranging from 3.82% to 9.52%, 11 QTLs were identified for the first branch height, located on A01, A03, A09, C01, and C03, respectively, with explained phenotypic variation ranging from 4.01% to 16.54%, four QTLs were identified for branch segment, located on A07, A09, C03, and C04, respectively, with explained phenotypic variation ranging from 4.79% to 8.10%, two QTLs were identified for average internode length, located on A07 and C05, respectively, with explained phenotypic variation ranging from 4.29% to 6.04%. Five of these QTLs were persistently expressed in different year environments or in different populations. These QTLs provide useful information for improving plant height in rapeseed breeding.