纤维素分解菌对堆肥有机质、全碳及纤维素降解率的影响

从堆肥、牛粪、秸秆堆腐物中分离4株纤维素分解菌。在纤维素粉及刚果红培养基中筛选出3株生长速度快、透明圈出现早且透明圈直径与菌落直径比值大的菌株,分别为FDQ1、FDQ2、FDQ3。不同菌株混合培养较单独培养酶活力提高,表明菌株间有协同作用。FDQ1＋FDQ2和FDQ1＋FDQ3菌株等比例混合进行牛粪与鸡粪混合堆肥,结束时有机质分别下降19.57%和18.40%,对照下降6.58%;全碳量分别为31.33%、32.42%,对照为41.20%,较发酵初期降低13.80%、12.71%和3.93%;纤维素分解率较对照提高95.12%和78.61%,表明纤维素分解菌在加快纤维素降解的同时还促进了碳素矿化。

Effect of Cellulose-Decomposing Microorganisms on Organic Matter ,Total Carbon and Degradation Rate of Cellulose

4 strains of cellulose-decomposing microorganisms were isolated from composting,cattle manure and straw filth.FDQ1,FDQ2 and FDQ3,which had fast growth rate,earlier transparent circle,bigger ratio of transparent circle diameter and colony diameter,were screened from cellulose and congo red medium.Enzyme activity of mixed culture was increased compared with single culture,which showed that strains had synergistic effect.Mixing FDQ1+FDQ2,FDQ1+FDQ3 with chicken and cattle manure in equal ratio,it showed that organic matter decreased by 19.57% and 18.40% after compost,the control decreased by 6.58%;total carbon contents were 31.33% and 32.42%,respectively,the control was 41.20%,which decreased by 13.80%,12.71% and 3.93% compared with the early stage of the fermentation;degradation rate of cellulose increased by 95.12% and 78.61% compared with the control.It was concluded that cellulose-decomposing microorganisms could acclerate cellulose degradation and promote carbon mineralization.