抗氧化剂在猪精液冷冻保存技术中的应用

近年来,在猪精液冷冻保存技术中应用抗氧化剂以提高冷冻精液质量的研究受到了广泛关注。国内外相继报道,添加甲基黄嘌呤、丁羟甲苯、谷胱甘肽、超氧化物歧化酶和过氧化物酶、维生素E及褪黑素等抗氧化剂可以有效改善冷冻保存猪精液的精子运动学参数,保护精子质膜、顶体和DNA完整性,提高冷冻-解冻后精子的受精能力。为了更好地了解抗氧化剂的抗精子冷冻损伤的作用机制、客观地评价不同抗氧化剂的应用效果及展望其在猪精液冷冻保存技术中的应用前景特综述如下。

Application of Antioxidant to the Cryopreservation Techniques of Boar Semen

The application of antioxidant to the cryopreservation techniques of boar semen attracted considerable research efforts which focused on defining how to improve quality of postthaw spermatozoa,recent years. The data revealed that the effect of supplemented with methyl xanthines, butylated hydroxytoluene, glutathione, superoxide dismutase and catalase, alpha- tocopherol or melatonin in extender on improving kinematics parameters, protecting sperm plasma membrane integrity, acrosome integrity and DNA integrity, and increasing the sperm fertilizability after freeze-thawing. This review will contribute to make a better understanding of these antioxidants on the mechanisms of resistance to sperm cryodamage, develop an objective evaluation of effectiveness and estimate the applied potential on the cryopreservation techniques of boar semen.