油松松子壳多糖超声波法提取佳工艺研究（英文）

目的]建立超声波热水浸提法提取油松松子壳多糖最佳工艺条件。方法]利用超声波热水浸提法,苯酚-浓硫酸法检测多糖含量,选择浸提温度、时间、固液比和浸提次数进行单因素梯度实验,再通过L9（34）正交实验优选出油松松子壳多糖提取最佳条件。结果]油松松子壳多糖最佳提取条件为浸提温度60℃,时间25 min,固液比1∶3.5,反复浸提2次。该方法所得回收率（n=5）在97.71%~100.67%之间,RSD为1.56%,稳定性较好。结论]该研究建立了一个简单高效的油松松子壳多糖提取方法,为废弃松子壳资源的开发利用奠定了坚实的基础。

Optimization of Ultrasound-assisted Extraction Conditions for Polysaccharides from Pinus tabulaeformis Seed Shells

Objective] This study aimed to investigate the optimal technological condi- tions for ultrasouqd-assisted extraction of polysaccharides from Pinus tabulaeformis seed shells. Melhod] Polysaccharides were extracted from P. tabulaeformis seed shells by ultrasound-assisted hot water extraction method and detected by phenol- concentrated sulfuric acid method. The effect of extraction temperature, extraction duration, solid-liquid ratio and extraction frequency on the extraction of polysaccha- rides was investigated using single-factor gradient experiment and the extraction conditions for polysaccharides from P. tabulaeformis seed shells were optimized us- ing L9（34） orthogonal design. Result] The content of polysaccharides extracted twice at 60 ℃ for 25 min with solid-liquid ratio of 1：3.5 reached the maximum. The re- covery rate （n=-5） ranged from 97.71% to 100.67% and RSD was 1.56%, suggesting high stability. Conclusion] A simple and efficient method for ultrasound-assisted ex- traction of polysaccharides from P. tabulaeformis seed shells was established in this study, which laid solid foundation for the development and utilization of abandoned pine nut shell resources.