微生物发酵烟梗水提物的制备及其在再造烟叶中的应用

为提高烟梗水提物的抽吸品质与可用性,采用从红大烟叶表面分离的HD4、HD12菌株对烟梗水提物进行发酵处理.经GC - MS检测,与未经处理的烟梗水提物(CK)相比,经徽生物处理后的烟梗水提物中香味物质的含量增加,其中,经HD4、HD12菌株处理后的烟梗水提物样品中香味物质总相对含量分别增加了12.3个百分点和18.7个百分点；杂环类物质种类和相对含量均明显增加,HD4菌株处理后的样品杂环类物质由6种增加到11种,相对含量由3.29％增加到40.63％；进行再造烟叶涂布后感官评价发现,使用微生物处理烟梗水提物的再造烟叶样品香气丰富性与香气量增加、木质杂气减少、细腻性与甜润感改善,抽吸品质有较大提升,且HD4样品的抽吸效果好于HD12样品.因此,使用微生物对烟梗水提物进行发酵处理是提高烟梗水提物抽吸品质与可用性的有效方法,且HD4菌株的效果较优.

Study on Preparation of Fermented Tobacco Stem Extracts and Application to Reconstituted Tobacco

Tobacco stem extracts were fermented by bacteria from Hongda tobacco to improve the sensory quality and application effect of the original extract.Analyzed by GC-MS,more flavors compared to the control extract(CK) were tested in the tobacco stem extracts fermented by bacteria,among which the extract samples fermented by HD4 strain and HD12 strain increased the flavors 12.3 percentage points and 18.7 percentage points,respectively.Heterocyclic substances were also raised significantly compared to the control sample.Types of heterocyclic substances were increased from six to eleven,and the proportion was increased from 3.29% to 40.63% in the sample fermented by HD4 strain.Reconstructed tobacco coating experiment showed that the reconstructed tobacco samples dealt with fermented tobacco stem extracts could obtain more aroma types and quantity.Additionally,this method could improve the smooth and sweet sense of tobacco as well as sensory quality.In contrast,the HD4 sample had a better sensory quality than HD12 sample.Overall,this is an effective way to increase the sensory quality and application effect of tobacco stem extracts under the fermentation by bacteria,especially by HD4 strain.