猪圆环病毒2型感染后猪血液学指标研究

为观察PCV2感染猪对其血常规及生化指标变化的影响,将6头PCV2抗体阴性的21日龄断奶仔猪随机分成攻毒组和对照组,攻毒组经颈部肌肉及口鼻分别接种PCV2-BH毒株,攻毒后检测部分血常规指标、免疫球蛋白质量浓度及部分血清生化指标。结果显示,感染后第4天和第7天,白细胞总数分别是正常生理水平的74.2%、79.9%;感染后第7天,淋巴细胞百分比是正常生理水平的35.05%,差异达显著水平(P<0.05),单核细胞和中性粒细胞百分比均显著低于对照组,说明PCV2感染降低了机体的先天免疫机能,增加了继发感染的几率,同时还说明PCV2-BH株具有较好的抗原性;攻毒组血清全部转阳,说明PCV2-BH毒株具有较好的免疫原性,可作为开发PCV2疫苗的候选毒株。

Study on Hematology Indexes in Pigs Infected with Porcine Circovirus Type 2

To observe the impact of the hematology indexes in pigs infected with porcine circovirus type 2(PCV2),6 weaned piglets with the PCV2 antibody negative were divided into two groups and the piglets in test group were inoculated with PCV2-BH strain by musculi colli,mouse and nose.The hematology indexes and anti-PCV2 antibody as the blood routine were detected post inoculation.The results showed that at 4 dpi and 7 dpi,the total white blood cells were 74.2% and 79.9% of the normal physiological level.At 7 dpi,the lymph-cell was 35.05% and both of the white blood cells and lymph-cell were significantly lower than the control group.The monocytes and neutrophils significantly lower than the control group at 7 dpi,suggesting that the innate immune function of the body was reduced by PCV2 infection.The PCV2 antibody in serum of the test group turned positive,showing that PCV2-BH strain had good immunogenicity,which provided the basis for developing vaccine of PCV2.