| 拟南芥AGL15基因的克隆及其植物超表达载体的构建 |
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| 引用本文： | 吴晓梅,刘忠丽.拟南芥AGL15基因的克隆及其植物超表达载体的构建[J].河南农业科学,2012,41(5):33-36. |
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| 作者姓名： | 吴晓梅 刘忠丽 |
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| 作者单位： | 1. 丽水学院生态学院,浙江丽水,323000
2. 浙江大学农业与生物技术学院/作物科学研究所,浙江杭州,310029 |
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| 摘 要： | 为了研究AGL15基因在种子发育过程中的作用,应用聚合酶链式反应技术(PCR)扩增了拟南芥AGL15基因,并将其克隆到pMD20-T vector载体上,对重组子进行PCR检测和限制性内切酶分析,并测定了该基因全序列。结果表明,该基因全长为807bp。将拟南芥AGL15基因定向克隆到植物表达载体pCambia1301的35S启动子下游,双酶切鉴定结果表明,AGL15基因在双元表达载体中的插入位置和方向都正确,即成功构建了该基因的植物超表达载体。
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| 关 键 词： | 拟南芥 PCR AGL15基因 超表达载体 |
Cloning and Construction of Plant Over-expression Vector Containing AGL15 Gene in Arabidopsis thaliana |
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| WU Xiao-mei
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LIU Zhong-li.Cloning and Construction of Plant Over-expression Vector Containing AGL15 Gene in Arabidopsis thaliana[J].Journal of Henan Agricultural Sciences,2012,41(5):33-36. |
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| Authors: | WU Xiao-mei LIU Zhong-li |
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| Institution: | 1.Ecology Department,Lishui College,Lishui 323000,China; 2.Institute of Crop Science/College of Agriculture and Bio-technology,Zhejiang University,Hangzhou 310029,China) |
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| Abstract: | In order to explore its functions during the seed development,AGAMOUS-Like15(AGL15) gene in Arabidopsis thaliana was obtained by PCR technique and cloned into pMD20-T vector.The recombinant clone was detected by PCR technique and analyzed by the restriction enzyme,and the full length of AGL15 was sequenced.The results showed that the length of the clone sequence was 807 bp.AGL15 gene in Arabidopsis thaliana was cloned into plant expression vector pCambia1301 in orientation,downstream of the promoter 35S.Double enzyme digestion results indicated that the position and orientation of AGL15 gene in pCambia1301 were exactly right,and the plant over-expression vector was constructed successfully. |
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| Keywords: | Arabidopsis thaliana PCR AGL15 gene over-expression vector |
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