基于RAPD技术的韭菜品种间遗传多样性分析

为了研究韭菜种质资源的多样性和品种间的相似问题,利用RAPD分子标记技术分析了我国11个地区15个韭菜品种间的遗传多样性。结果表明,采用20条RAPD引物,共扩增出136个条带,其中多态性位点98个,平均多态性比率为72%。依据15个韭菜品种间的Nei’s遗传距离进行UPGMA聚类分析,在遗传距离为0.34处将15个韭菜品种分为4个类群:第1类群包含独根红、兰州白根和南京马鞭韭3个品种;第2类群包含汉中冬韭、上海强韭-3和上海强韭-6 3个品种;第3类群包含四季青、桂林小红根、天津大青苗、新疆白根和嘉兴雪莲5个品种;第4类群包含天津大金钩、环韭、平丰苔韭和抗寒薹韭4个品种。韭菜品种间遗传相似性和来源地之间没有必然的联系。

Analysis of Genetic Diversity in Leek Cultivars Based on RAPD

Random amplified polymorphic DNA(RAPD) markers were used to estimate the genetic diversity in 15 cultivars of Allium tuberosum(leek) from 11 regions in China.Twenty random primers generated a total of 136 reproducible and discernible loci,among which 98 were polymorphic with the percent of polymorphic loci of 72%.Based on the Nei’s genetic distances between the 15 cultivars,UPGMA cluster analysis was performed and showed that all cultivars were classified into four groups at genetic distance of 0.34,i.e.,Goup 1 contained 3 cultivars(Du Genhong,Lanzhou baigen and Nanjing mabianjiu);Goup 2 included 3 cultivars(Hanzhong dongjiu,Shanghai qiangjiu-3 and Shanghai qiangjiu-6);Goup 3 contained 5 cultivars(Sijiqing,Guilin xiaohonggen,Tianjin daqingmiao,Xinjiang baigen and Jiaxing xuelian);Goup 4 included 4 cultivars(Tianjin dajingou,Huanjiu,Pingfeng taijiu and Kanghan taijiu).There were no correlation between cultivars and origin regions of leeks.