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| IBDV VP2蛋白P22表位多肽的原核表达及初步鉴定 | |
| 引用本文： | 王倩倩,张改平,王选年,宋幸辉,卢清侠,王世红,王爱萍.IBDV VP2蛋白P22表位多肽的原核表达及初步鉴定[J].河南农业科学,2012,41(5):150-153. |
| 作者姓名： | 王倩倩 张改平 王选年 宋幸辉 卢清侠 王世红 王爱萍 |
| 作者单位： | 1. 郑州大学生物工程系,河南郑州,450001 2. 河南省农业科学院农业部动物免疫学重点开放实验室/河南省动物免疫学重点实验室,河南郑州,450002 3. 河南省农业科学院农业部动物免疫学重点开放实验室/河南省动物免疫学重点实验室,河南郑州450002;新乡学院生物技术研究中心,河南新乡453003 |
| 基金项目： | 国家自然科学基金，河南省科技创新杰出人才项目 |
| 摘 要： | 为了进一步了解传染性法氏囊病毒(IBDV)VP2蛋白P22多肽抗原表位的功能,根据IBDVVP2蛋白的三维结构,设计合成编码P22多肽的基因序列,通过大肠杆菌密码子优化,并将基因序列P22串联二次合成并转化大肠杆菌BL21(DE3)菌株。用IPTG诱导P22基因的表达。表达纯化后的P22多肽经SDS-PAGE电泳和Western-blot分析及传染性法氏囊病毒快速检测试纸条检测。结果表明,P22表位多肽的分子质量约为16kD,并能被His单抗识别,用试纸条检测纯化的P22多肽,呈阳性反应结果。提示IBDV VP2蛋白P22多肽能与IBDV单抗特异性反应。 |
| 关 键 词： | 法氏囊病毒 P22抗原表位 SDS-PAGE 多肽 检测 |
Prokaryotic Expression and Identification of P22 Antigen Peptide on VP2 Protein of Infectious Bursal Disease Virus |
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| WANG Qian-qian , ZHANG Gai-ping , WANG Xuan-nian , SONG Xing-hui , LU Qing-xia , WANG Shi-hong , WANG Ai-ping.Prokaryotic Expression and Identification of P22 Antigen Peptide on VP2 Protein of Infectious Bursal Disease Virus[J].Journal of Henan Agricultural Sciences,2012,41(5):150-153. | |
| Authors: | WANG Qian-qian ZHANG Gai-ping WANG Xuan-nian SONG Xing-hui LU Qing-xia WANG Shi-hong WANG Ai-ping |
| Institution: | 1*(1.Department of Bioengineering,Zhengzhou University,Zhengzhou 450001,China; 2.Key Laboratory of Animal Immunology of the Ministry of Agriculture,Henan Provincial Key Laboratory of Animal Immunology,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China; 3.Biotechnology Research Center,Xinxiang University,Xinxiang 453003,China) |
| Abstract: | In order to study the function of P22 peptide on the VP2 protein of infectious bursal disease virus(IBDV),the sequence of P22 peptide was designed according to three dimensional structures of VP2 on IBDV.P22 peptide was synthesized and expressed in E.coli BL21(DE) strain.After purification and renaturation,P22 peptide was analyzed by SDS-PAGE,Western-blot and IBDV rapid diagnostic strip.The results showed that the molecular weight of P22 peptide is about 16 kD and can be detected by anti-His monoclonal antibody.IBDV rapid diagnostic strip can interact with P22 peptide,indicating that anti-IBDV monoclonal antibody can detect P22 peptide specifically. |
| Keywords: | IBDV P22 antigen epitope SDS-PAGE peptide detection |
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