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| 双色茉莉茎段离体培养再生植株的研究 | |
| 引用本文： | 王玲平,魏海龙,石大兴,王米力.双色茉莉茎段离体培养再生植株的研究[J].浙江林业科技,2008,28(5). |
| 作者姓名： | 王玲平 魏海龙 石大兴 王米力 |
| 作者单位： | 1. 浙江省农业科学院蔬菜研究所,浙江,杭州,310021 2. 浙江省林业科学研究院,浙江,杭州,310023 3. 四川农业大学,四川,雅安,625014 |
| 摘 要： | 以双色茉莉茎段为外植体,探讨外植体取材季节和部位、基本培养基种类、植物生长调节剂组合、活性炭等对茎段器官发生和植株再生的影响.结果表明:在4月和10月取材最好,其诱导率分别达到了92.780/和84%,污染率最低;1/2MS 0.01 mg/L NAA 3.0 mg/L BA 2.0 mg/L KT培养基最适合不定芽的诱导分化,不定芽分化率达到了88.34%;在继代增殖培养中,继代次数最好在6代以内,否则增殖率下降,MS 0.5mg/L IBA 3.0mg/LBA 0.2%活性炭培养基最适合不定芽的增殖(5.92)和大于2cm芽的分化(47.16%).不定芽可在1/2MS培养基中有效伸长,适宜的生根培养基为1/2MS 1.0 mg/L IBA 0.2 mg/L BA,生根率达到100%. |
| 关 键 词： | 双色茉莉 茎段 离体培养 组织培养 再生植株 |
In Vitro Plant Regeneration From Brunfelsia latifolia Stem |
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| WANG Ling-ping,WEI Hai-long,SHI Da-xing,WANG Mi-li.In Vitro Plant Regeneration From Brunfelsia latifolia Stem[J].Journal of Zhejiang Forestry Science and Technology,2008,28(5). | |
| Authors: | WANG Ling-ping WEI Hai-long SHI Da-xing WANG Mi-li |
| Institution: | WANG Ling-ping1,WEI Hai-long2,SHI Da-xing3,WANG Mi-li3 |
| Abstract: | Study was made on effect of stem collection season and part,basic medium,regulator combinations and activated carbon on in vitro plant regeneration of Brunfelsia latifolia.The result showed that shoot induction rate could reach 92.78% and 84% respectively with the lowest pollution rate by the stems collected for explant in April and October.1/2MS+0.01mg/L NAA+3.0mg/L BA+2.0mg/L KT was the optimum medium for adventitious bud induction,topping 88.34% of differentiation ratio.The optimum medium for bud prolife... |
| Keywords: | Brunfelsia latifolia stem culture in vitro plant regeneration |
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