| 应用纳米磁珠快速检测芒果果核象甲Application of magnetic nanoparticles for rapid identification of Sternochetus mangifera |
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| 作者姓名： | 刘若思 张丽杰 赵晓丽 张仑 邓丛良 |
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| 作者单位： | 1.北京出入境检验检疫局技术中心,北京 101312;2.中国农业大学农学与生物技术学院,北京 100193;3.重庆大学生命科学院,重庆 401331 |
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| 摘 要： | 利用纳米磁珠(magnetic nanoparticles, MNP)的磁性分离特性,结合PCR技术,建立一种简单方便的芒果果核象甲Sternochetus mangiferae检测新方法。该方法可以较简便的提取出昆虫的DNA,且用时仅10 min左右,相对普通DNA提取试剂盒效率明显提升。Using the magnetic separation of magnetic, nanoparticles (MNP), in combination with PCR technique, a simple and convenient method was established to identify Sternochetus mangiferae. The results showed that using this method, the DNA of S. mangiferae can be extracted in 10min, and compared with using regular DNA extraction kit, the detection efficiency was improved significantly.
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| 关 键 词： | 芒果果核象甲 纳米磁珠 检疫性有害生物 快速鉴定Sternochetus mangiferae Magnetic nanoparticles Quarantine pest Rapid identification |
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