香雪兰种子胚的组织培养和植株再生

香雪兰的成熟胚和未成熟胚切段，在含有ＩＡＡ２ｍｇ／Ｌ、ＮＡＡ０３ｍｇ／Ｌ和ＢＡＰ０５ｍｇ／Ｌ的改良Ｎ６培养基上可形成半透明的瘤状愈伤组织。将这种愈伤组织培养于含有ＮＡＡ０３ｍｇ／Ｌ、ＢＡＰ０５ｍｇ／Ｌ和ＫＴ０５ｍｇ／Ｌ的ＭＮ６培养基可分化出丛生芽。生根培养基为含ＮＡＡ０５ｍｇ／Ｌ的改良Ｎ６培养基。相同的外植体可在ＭＮ６＋ＩＡＡ２０ｍｇ／Ｌ＋ＢＡＰ３０ｍｇ／Ｌ培养基上通过体细胞胚胎发生途径直接形成小植株。讨论了外源激素对香雪兰种子胚离体培养的作用。

Tissue Culture of Embryo and Regeneration of Plant in Freesia refracta Klatt.

Some translucent nodular calli were formed from segments of mature and immature embryos of Freesia refracta on the modified N 6 medium with 2mg/L IAA,0.3mg/L NAA and 0.5mg/L BAP. Some buds were appeared on the calli which were transferred to the MN 6 medium containing 0 3mg/L NAA, 0 5mg/L BAP and 0 5mg/L KT. The MN 6 medium with 0 5mg/L NAA was used for rooting. When segments of embryo explant were cultured on the medium containing 2mg/L IAA and 3mg/L BAP, plantlets were regenerated directly via the somatic embryogenesis. In this report, the role which exogenous hormones played in the tissue culture of embryo was also discussed.