| 锦丰梨花粉植株的诱导 |
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| 引用本文： | 薛光荣,杨振英.锦丰梨花粉植株的诱导[J].园艺学报,1996,23(2):123-127. |
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| 作者姓名： | 薛光荣 杨振英 |
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| 作者单位： | 中国农业科学院果树研究所 辽宁兴城125100
(薛光荣，杨振英，史永忠，方成泉)，中国农业科学院果树研究所 辽宁兴城125100(贾敬贤) |
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| 摘 要： | 采用花粉发育单核期的‘锦丰’梨花药,接种在1/2 MS附加IAA 0.2mg/L、BA1～2mg/L的培养基上,经过120天产生胚状体。胚状体转入MS附加GA3 0.1mg/L、IBA 0.2mg/L、BA 1mg/L的分化培养基上,经过85天分化幼梢。无根植株转入1/2 MS附加IAA 1.5mg/L的培养基上,经过14天诱导生根,移栽和嫩枝嫁接成活情况良好。
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| 关 键 词： | 梨 花药培养 胚状体 分化植株 移栽 嫩枝嫁接 |
Induction of Pollen Plantlets by Anther Culture in Vitro in' Jin Feng' Pear |
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| Xue Guangrong,Yang Zhenying,Shi Yongzhong,Fang Chengquan and Jia Jingxian.Induction of Pollen Plantlets by Anther Culture in Vitro in'' Jin Feng'' Pear[J].Acta Horticulturae Sinica,1996,23(2):123-127. |
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| Authors: | Xue Guangrong Yang Zhenying Shi Yongzhong Fang Chengquan and Jia Jingxian |
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| Institution: | Xue Guangrong,Yang Zhenying,Shi Yongzhong,Fang Chengquan* and Jia Jingxian |
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| Abstract: | The embryoids were induced by inoculating uninucleate anther of pear (cv. Jin Feng) on 1 / 2 MS medium supplemented with IAA 0.2mg/ L and BA 1-2mg/ L for 120 days. The rootless plantlets differentiated from embryoids transferred on Ms medium supplemented with GA3 0.1mg/ L, IB A 0.2mg/ L, and BA 1mg/ L for 85 days. The plantlets with roots were induced from rootless plantlets transferred on 1 / 2 MS medium supplemented with IAA 1.5mg/ L for 14 days. The anther plantlets of Jin Feng' pear were succesfully planted and grafted in the field. |
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| Keywords: | Pear Anther culture Embryoid Plantlets differentiation Transplanting Grafting |
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