全价配合饲料条件下克氏原螯虾幼体及成体消化酶的活力研究

目的]探讨全价配合饲料条件下克氏原螯虾幼体及成体消化酶的活性。方法]取克氏原螯虾幼体全消化道组织,成体胃、肠组织,对克氏原螯虾蛋白酶、淀粉酶、脂肪酶、纤维素酶等消化酶活性进行测定。结果]克氏原螯虾幼体的淀粉酶活性较高,胃蛋白酶活性较低;纤维素酶和脂肪酶活性相差不大,且活性均小于淀粉酶活性。成体的胃和肠中蛋白酶及淀粉酶比活力均较高,而纤维素酶和脂肪酶的比活力较低,且蛋白酶比活力为胃大于肠,淀粉酶比活力为肠略大于胃,纤维素酶和脂肪酶比活力在胃和肠中无明显差异。结论]该研究为克氏原螯虾的水产养殖中专用饲料的开发提供了参考资料。

Study on Activities of Digestive Enzymes of Procambarus clarkii in Adult and Larvae Under Complete Formula Feed

Objective] The aim was to discuss the activities of digestive enzymes of Procambarus clarkii in adult and larvae under complete formula feed.Method]Collecting the whole digestive tract tissues of P.clarkii larvae and stomach tissue and intestinal tissue of adult P.clarkii,the activities of digestive enzymes such as protease,amylase,lipase and cellulase were detected.Result] The amylase activity in larvae of P.clarkii was higher,while the stomach protease activity was lower.The difference between the activities of cellulase and lipase of P.clarkii larvae was small,and the activities of cellulase and lipase were all lower than that of amylase.The activities of protease in stomach and intestine of adult P.clarkii and amylase were all higher,while that of cellulase and lipase were lower,and the protease activity in stomach was higher than that in intestine,amylase activity in intestine was little higher than that in stomach,the activities of cellulase and lipase had no difference in stomach or in intestine.Conclusion] The research provided the reference data for the development of special feed in aquaculture of P.clarkii.