| 厚壳贻贝血细胞颗粒的蛋白质组学分析 |
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| 引用本文： | 王信超,孙敬敬,范美华,武梅,石戈,王日昕,廖智. 厚壳贻贝血细胞颗粒的蛋白质组学分析[J]. 南方水产, 2012, 8(2): 7-14 |
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| 作者姓名： | 王信超 孙敬敬 范美华 武梅 石戈 王日昕 廖智 |
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| 作者单位： | 浙江海洋学院海洋科学学院,海洋生物资源及分子工程实验室,浙江舟山316004 |
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| 基金项目： | 浙江省科技厅面上科研农业项目 |
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| 摘 要： | 贝类的血细胞在宿主的免疫防御机制中发挥着重要的作用。文章利用线性离子阱-四级杆质谱对厚壳贻贝（Mytilus coruscus）血细胞的颗粒蛋白质组分进行了Shotgun分析,结合Mascot数据库搜索,共鉴定了125种高丰度蛋白,其中包括细胞骨架蛋白、细胞粘附蛋白、精氨酸激酶和代谢相关酶类等。此结果为深入了解厚壳贻贝血细胞颗粒的蛋白组成、贻贝免疫分子标记的筛选及其免疫机制奠定了基础。
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| 关 键 词： | 厚壳贻贝 血细胞颗粒 LTQ—Orbitrap 质谱 Shotgun |
Proteomic analysis of granule of hemocytes from Mytilus coruscus |
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| WANG Xinchao , SUN Jingjing , FAN Meihua , WU Mei , SHI Ge , WANG Rixin , LIAO Zhi. Proteomic analysis of granule of hemocytes from Mytilus coruscus[J]. South China Fisheries Science, 2012, 8(2): 7-14 |
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| Authors: | WANG Xinchao SUN Jingjing FAN Meihua WU Mei SHI Ge WANG Rixin LIAO Zhi |
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| Affiliation: | (Lab. of Marine Biological Source and Molecular Engineering, College of Marine Sciences, Zhejiang Ocean University, Zhoushan 316004, China) |
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| Abstract: | The blood cells of mussel play an important role in the host＇s immune defense mechanism. We explore the protein composi- tion in the granule of hemocytes from Mytilus coruscus by using liuear-ion-trap-quadrupole mass spectrometry （LTQ-Orbitrap） combined with Shotgun strategy. A total of 125 high-abundance proteins are identified by Mascot searching methods, including cytoskeletal pro- teins, cell adhesion proteins, arginine kinases and metabolism-related enzymes, etc.. The results lay a foundation for further studies on the composition of mussel protein particles, screening of its immune molecular markers as well as its immune mechanism. |
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| Keywords: | Mytilus coruscus hemocytes granule LTQ-Orbitrap mass spectrometry Shotgun |
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