A skeletal linkage map of Hordeum bulbosum L. and comparative mapping with barley (H. vulgare L.) |
| |
Authors: | B. Jaffé P.D.S. Caligari J.W. Snape |
| |
Affiliation: | (1) Cereals Research Department, John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, U.K.;(2) Department of Agricultural Botany, The University of Reading, Reading |
| |
Abstract: | A restriction fragment length polymorphism (RFLP) based linkage map of a cross between two diploid Hordeum bulbosum (2n = 2x = 14) clones, PB1 and PB11, was constructed from 46 recombinant progeny clones. Since both parents are heterozygous, separate and combined parental maps were constructed. All of the RFLP markers screened had previously been mapped in barley (H. vulgare L.) so that comparative maps could be produced. The PB1 linkage map consists of 20 RFLP marker loci assigned to four linkage groups covering 94.3 cM. The PB11 linkage map consists of 27 RFLP marker loci assigned to six linkage groups covering 149.1 cM. Thirteen markers polymorphic in both parents were used as ‘anchors’ to create a combined linkage map consisting of 38 loci assigned to six linkage groups and covering a genetic distance of 198 cM. Marker order was highly conserved in a comparison with the linkage map of H. vulgare (Laurie etal., 1995). However, in contrast, the genetic distances for the same markers were very different being 649 cM and 198 cM respectively, a genetic distance ratio of 1: 3.3. Thus although the map was short, it can be presumed to cover half the genome of H. bulbosum. This study provides further confirmation of the close relationship between the two species and gives a basis for the development of marker mediated introgression through interspecific hybridisation between the two species. This revised version was published online in July 2006 with corrections to the Cover Date. |
| |
Keywords: | Comparative genetics Hordeum bulbosum Hordeum vulgare mapping RFLP |
本文献已被 SpringerLink 等数据库收录! |
|