大孔树脂分离纯化南姜黄酮类化合物的研究

本文以大孔吸附树脂为吸附剂,研究其对南姜黄酮类化合物的吸附分离特性,选择FL-1、FL-2、FL-3、AB-8四种大孔吸附树脂,比较其对南姜黄酮类化合物的吸附率和解吸率,筛选出最佳树脂,并对其动力学曲线和静态吸附性能进行了考察。结果表明,FL-3树脂对南姜黄酮类化合物有较好的吸附和解吸效果。当上样液质量浓度为0.314 mg/m L,pH=4,上样液流速为1 m L/min时,FL-3树脂对南姜黄酮类化合物吸附量最大;以pH=5的80%乙醇为洗脱剂,洗脱液流速为1.5 m L/min时,解吸效果最好,达93.2%;经纯化后南姜黄酮类化合物纯度为40.3%,达到良好的分离纯化效果。

Determination and Stability Analysis of Anthocyanidin in Three Kinds of Fruit Peels

In order to select the best macroporous resin, and investigate their kinetics curve and static adsorption performance, the characteristics of adsorption and separation of four types of macroporous resin including FL-1, FL-2, FL-3, AB-8 were compared on their adsorption and desorption rates for purifing flavonoids from Alpinia galangal (L.) Willd in this paper. The results showed that, FL-3 resin had higher adsorption and desorption capacity, it exhibited best adsorption performance when loading buffer concentration was 0.314 mg/mL, pH value was 4 and the flow velocity was 1 mL/min. And when eluant ethyl alcohol content was 80%, pH value was 5, the flow velocity was 1.5 mL/min, the largest desorption quantity of FL-3 macroporous resin reached 93.2%. Meanwhile, the purity of flavonoids was up to 40.3%. It can be used to purify and separate flavonoids from Alpinia galangal (L.) Willd.