枯斑盘多毛孢Pf-毒素活性组分的分离纯化

 对枯斑盘多毛孢(Pestalotia funerea)培养滤液用70%乙醇沉淀,将其分为可溶性和不可溶(蛋白质等大分子)组分,浸渍法活性检测表明,蛋白质等大分子物质对马尾松、湿地松切根幼苗没有致病活力,非蛋白部分(Pf-毒素)则保留了病原菌培养滤液的致病活性,且与病原菌培养滤液相比活性有所增强。以最适流动相,正丁醇:甲醇:水(4:1:2)为洗脱液,采用硅胶H60型柱反复柱层析,从枯斑盘多毛孢的甲醇粗提物质中分离出9种组分,经致病活性测定出3种组分,Rf值分别为0.83、0.79、0.80,它们对马尾松切根幼苗、湿地松切根幼苗针叶都有致萎作用。

Isolation and purification of Pf-toxin from Pestalotia funerea

Culture filter of Pestalotia funerea was primarily fractioned by enthanol precipitation. High molecular weight substances, such as the proteins, were shown to be nonpathogenic to the cut-root seedling of Pinus massoniana and P. el- liotti by soaking method, whereas the non-protein paxt was pathogenic. Nine fractions separated by gel H60 column chro- matography using n-butanol: methanol: water (4: 1: 2) as mobile phase were assayed. Among them, three fractions with Rf values of 0.83, 0.79, 0.80 were found to be pathogenic to the leaves of P. massoniana and P. elliotti.