| 农杆菌介导的玉米大斑病菌的遗传转化 |
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| 引用本文： | 刘建光,王艳辉,张国珍,贾慧,董金皋. 农杆菌介导的玉米大斑病菌的遗传转化[J]. 植物病理学报, 2007, 37(6): 675-678 |
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| 作者姓名： | 刘建光 王艳辉 张国珍 贾慧 董金皋 |
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| 作者单位： | 1 河北农业大学生命科学学院, 保定 071001;2 中国农业大学植物病理学系, 北京 100094 |
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| 摘 要： | 玉米大斑病是玉米生产上的一种重要真菌病害,在世界主要玉米产区都曾造成过严重的经济损失。目前,有关该病原菌的生理分化、遗传变异及毒素产生等方面研究报道较多,而在玉米与大斑病菌互作的分子机理方面研究相对较少。
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| 关 键 词： | 玉米大斑病菌 农杆菌介导转化 遗传转化 真菌病害 原生质体转化 丝状真菌 转化效率 突变体库 |
| 文章编号： | 0412-0914（2007）06-0675-04 |
| 收稿时间： | 2006-10-24 |
| 修稿时间： | 2007-04-18 |
Agrobacterium tumefaciens-mediated transformation of Setosphaeria turcica |
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| LIU Jian-guang,WANG Yan-hui,ZHANG Guo-zhen,JIA Hui,DONG Jin-gao. Agrobacterium tumefaciens-mediated transformation of Setosphaeria turcica[J]. Acta Phytopathologica Sinica, 2007, 37(6): 675-678 |
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| Authors: | LIU Jian-guang WANG Yan-hui ZHANG Guo-zhen JIA Hui DONG Jin-gao |
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| Affiliation: | 1 College of Life Sciences, Agricultural University of Hebei, Baoding 071001, China;2 Department of Plant Pathology, China Agricultural University, Beijing 100094, China |
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| Abstract: | Agrobacterium tumefaciens-mediated transformation(ATMT) is a useful method in genetic transformation of a wide range from plant species,yeasts,fungi to human cells.In this paper,the Setosphaeria turcica had been successfully transformed by using the ATMT system with an efficiency at 40-60 transfor-mants per 105 spores.PCR and Southern blot analysis of the transformants showed that the T-DNA had been integrated into genome of the fungus by single copy at random sites.ATMT of S.turcica provides an opportunity for advancing studies of the molecular genetics of the fungus and the molecular mechanism of its pathogenicity to maize. |
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| Keywords: | Setosphaeria turcica T-DNA Agrobacterium tumefaciens-mediated transformation |
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