Improved multiple rapid immunofilter paper assay to detect three viruses of cucumber simultaneously |
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Authors: | Hideki Osaki Koji Nomiyama Koichi Ishikawa |
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Institution: | (1) National Agricultural Research Center for Western Region, 6-12-1 Nishifukatsu, Fukuyama Hiroshima, 721-8514, Japan;(2) Shikoku Research Center, National Agricultural Research Center for Western Region, 1-3-1 Senyuu, Zentsuuji Kagawa, 765-8508, Japan |
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Abstract: | We improved a multiple rapid immunofilter paper assay (multi-RIPA) to simultaneously detect three major viruses of cucumber,
Cucumber mosaic virus, Melon yellow spot virus and Zucchini yellow mosaic virus. When we increased the concentration of bovine serum albumin (BSA) in the extraction buffer, a positive band for each virus
developed in the multi-RIPA, regardless of the order that the three sensitized white latex particles were immobilized on the
strip. The increase in BSA concentration also strengthened the sensitivity of each RIPA. In addition, using a mixture of commercially
available, red and blue, sensitized latex solutions as a tracer, purple bands could be produced on the strips. Because each
virus yielded an unique color for a positive reaction, the three viruses were easy to distinguish. |
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