桃小食心虫基因组DNA提取方法比较研究

以桃小食心虫(Carposina niponensis Walsingham)为材料,分别采用CTAB法,冰KAc法,SDS-PK法和试剂盒法提取桃小食心虫的基因组DNA.通过电泳、紫外光谱分析和ISSR-PCR扩增等检测手段,比较分析了DNA纯度.结果表明:CTAB法和SDS-PK法提取的DNA质量明显优于冰KAc法和试剂盒法,适用于PCR扩增.与其他方法比较,SDS-PK法提取的DNA经过PCR扩增后得到的多态性位点最多.因此,SDS-PK法是实验室提取桃小食心虫基因组有效、经济实用的方法.

Comparison on Methods for Extraction of Genomic DNA of Carposina niponensis

Genomics DNA were extracted from Carposina niponensis Walsingham using CTAB,KAc,SDS-PK,and genomic DNA kit method,respectively.DNA was detected by electrophoresis,UV and ISSR-PCR.The results suggested that,the quality of DNA samples extracted by CTAB method and SDS-PK method was obviously better and more suitable for PCR than that by the other two methods.In contrast with other methods,SDS-PK method presented the most polymorphic sites.Therefore,the SDS-PK method was the most effective,practical and economi...