| 百合鳞茎蔗糖合成酶活性检测体系的建立 |
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| 引用本文： | 孙红梅,王微微,何玲,王春夏,李天来. 百合鳞茎蔗糖合成酶活性检测体系的建立[J]. 沈阳农业大学学报, 2011, 42(3) |
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| 作者姓名： | 孙红梅 王微微 何玲 王春夏 李天来 |
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| 作者单位： | 沈阳农业大学园艺学院/设施园艺省部共建教育部重点实验室/辽宁省设施园艺重点实验室,沈阳,110161 |
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| 基金项目： | 国家自然科学基金项目(30972023); 中国博士后科学基金项目(20090451280); 辽宁省重点实验室项目(LS2010148) |
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| 摘 要： | 为了建立富含多糖的百合鳞茎蔗糖合成酶(sucrose synthase,EC2.4.1.13,SuSy)活性检测体系,深入研究其蔗糖代谢机制,以兰州百合(Lilium davidii var.unicolor)鳞茎外层鳞片为试材,分别研究了提取缓冲液种类及pH值、反应温度、底物浓度以及缓冲液pH值对SuSy合成和分解方向活性的影响.结果表明:SuSy合成方向活性检测的最适提取缓冲液是pH值为7.8的TrisHCl,最适反应温度为50℃,底物果糖最适浓度为50 mmol· L-1,UDPG最适浓度为5 mmol·L-1,反应缓冲液Tris-HCl最适pH值为7.5；SuSy分解方向活性检测的最适提取缓冲液为pH值7.8的Hepes-NaOH,最适反应温度为40℃,底物蔗糖最适浓度为10mmol· L-1,UDP最适浓度为7 mmol·L-1,反应缓冲液Mes-NaOH最适pH值为4.5.
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| 关 键 词： | 百合 鳞茎 蔗糖合成酶 提取条件 反应条件 |
Establishment of Detection System for Sucrose Synthase Activity in Lily Bulb |
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| SUN Hong-mei,WANG Wei-wei,HE Ling,WANG Chun-xia,LI Tian-lai. Establishment of Detection System for Sucrose Synthase Activity in Lily Bulb[J]. Journal of Shenyang Aricultural University, 2011, 42(3) |
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| Authors: | SUN Hong-mei WANG Wei-wei HE Ling WANG Chun-xia LI Tian-lai |
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| Affiliation: | SUN Hong-mei,WANG Wei-wei,HE Ling,WANG Chun-xia,LI Tian-lai (College of Horticulture/Horticulture Key Laboratory of Ministry of Education/Key Laboratory of Protected Horticulture of Liaoning Province,Shenyang Agricultural University,Shenyang 110161,China) |
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| Abstract: | This investigation was designed to establish the detection system for sucrose synthase(EC 2.4.1.13,SuSy) activities in lily bulb enriched with polysaccharides,which provided a detection method for the further research on the mechanism of sucrose metabolism.The effects of extracting buffer types,pH,reaction temperature,substrate concentrations,pH of the reaction buffer on the SuSy activities in both synthesis and decomposition direction were respectively studied by using the exterior scales of Lilium davidii... |
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| Keywords: | Lilium bulb sucrose synthase extraction condition reaction condition |
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