湖南首次检测到黄瓜绿斑驳花叶病毒

 黄瓜绿斑驳花叶病毒（Cucumber green mottle mosaic virus,CGMMV）自1935年首次报道^［1］以来,已广泛分布于亚洲、欧洲、南美洲的多个国家和地区,对葫芦科作物的生产造成了严重损失。CGMMV主要有两种重要的传播途径,一是带毒种子造成远距离传播,二是授粉、嫁接等农事操作造成的田间传播^［2］。

First report of Cucumber green mottle mosaic virus(CGMMV)in Hunan Province

Five leaf samples from watermelon plants with mosaic symptom in Shaoyang County were proved to be CGMMV-positive by RT-PCR detection using one pair of specific primers. The sequence of RT-PCR product (CGMMV-SY) was submitted to GenBank and showed identical to those of CGMMV coat protein genes in nucleotide databases after BLAST. The seeds of a rootstock variety widely used from Beijing were also detected CGMMV-positive and the RT-PCR product (CGMMV-JX) was of the same sequence as CGMMV-SY. They shared 100% nucleotide identities with some CGMMV isolates of Liaoning and Korea. It suggested that CGMMV occurred in watermelon crop in Hunan, might be originally transmitted by grafting with rootstock infected with CGMMV.