| 烟草靶斑病菌菌丝融合群及ITS序列分析 |
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| 引用本文： | 吴元华,伏颖,赵秀香,穆凌霄,赵艳琴.烟草靶斑病菌菌丝融合群及ITS序列分析[J].植物病理学报,2013,43(2):215-218. |
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| 作者姓名： | 吴元华 伏颖 赵秀香 穆凌霄 赵艳琴 |
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| 作者单位： | 沈阳农业大学植物保护学院,沈阳 110866 |
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| 基金项目： | 国家烟草专卖局科技项目“全国烟草有害生物调查研究”（国烟办综(2010)182号）;辽宁省烟草专卖局科技攻关项目“辽宁省烟草有害生物调查研究”（辽烟计(2010)86号） |
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| 摘 要： | 烟草靶斑病是2006年我国新报道发生的一种叶部病害［1］,其病原的无性世代为立枯丝核菌(Rhizoctonia solani Kühn),有性世代为瓜亡革菌(Thanatephorus cucumeris (Frank)Donk)。该病菌主要危害叶部形成病斑,对烟草的产量和品质影响显著,目前该病害主要分布在辽宁省丹东和铁岭地区,并呈现出迅速蔓延趋势。烟草靶斑病最早由巴西报道,此后,哥斯达黎加、美国、南非和津巴布韦也相继发生［2,3］。
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| 关 键 词： | Tobacco target spot Rhizoctonia solani K黨n anastomosis groups ribosomal DNA internal transcribed spacer sequence analysis |
| 收稿时间： | 2012-01-29; |
The anastomosis groups and ITS sequence analysis of Rhizoctonia solani isolates of tobacco target spot |
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| WU Yuan-hua
,
FU Ying
,
ZHAO Xiu-xiang
,
MU Ling-xiao
,
ZHAO Yan-qin.The anastomosis groups and ITS sequence analysis of Rhizoctonia solani isolates of tobacco target spot[J].Acta Phytopathologica Sinica,2013,43(2):215-218. |
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| Authors: | WU Yuan-hua FU Ying ZHAO Xiu-xiang MU Ling-xiao ZHAO Yan-qin |
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| Institution: | College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China |
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| Abstract: | Fifty-eight isolates of Rhizoctonia solani were isolated from tobacco target spot disease samples collected from tobacco growing areas in Liaoning Province. The test results of anastomosis groups (AGs) by glass slide fix position mating method showed that all isolates of R. solani belonged to AG-3. The rDNA ITS sequence of the R. solani strain YC-9 was amplified and sequenced by using the universal primers ITS1and ITS4, and compared by nucleotide BLAST with other nucleotides in GenBank. It was showed that the ITS sequence of YC-9 (653 bp) was completely identical to that of the R. solani AG-3 isolate from tomato foliar blight. The results provide important basis for further investigation of tobacco target spot disease. |
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