| 猪链球菌2型江苏分离株溶血素基因的检测及序列分析 |
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| 引用本文： | 方绍庆,陆承平. 猪链球菌2型江苏分离株溶血素基因的检测及序列分析[J]. 中国畜牧兽医, 2006, 33(3): 59-61 |
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| 作者姓名： | 方绍庆 陆承平 |
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| 作者单位： | 南京农业大学,南京,210095 |
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| 基金项目： | 国家973项目子课题(G1999011906) |
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| 摘 要： | 根据猪链球菌2型(strep tococcus su is type 2)溶血素基因(sly)设计和合成了一对可扩增其完整阅读框的引物,对HA 9801等6株猪链球菌2型江苏分离株、1株德国分离株SS2-D及猪链球菌C群参考株ATCC 35246的核酸进行PCR扩增,结果显示HA 9801等6株江苏分离株及德国株SS2呈阳性,ATCC 35246呈阴性。HA 9801株PCR产物纯化后测序,序列分析结果表明该DNA片段与猪链球菌2型1933株的sly基因同源性为99%。
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| 关 键 词： | 猪链球菌2型 溶血素 核苷酸序列 |
| 文章编号： | 1671-7236（2006）03-0059-03 |
| 收稿时间： | 2005-08-05 |
| 修稿时间： | 2005-08-05 |
Detection and Sequence Analysis of the Gene Encoding Suilysin from Streptcoccus suis type 2 Strains Isolated in Jiangsu Province |
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| FANG Shao-qing,LU Cheng-ping. Detection and Sequence Analysis of the Gene Encoding Suilysin from Streptcoccus suis type 2 Strains Isolated in Jiangsu Province[J]. China Animal Husbandry & Veterinary Medicine, 2006, 33(3): 59-61 |
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| Authors: | FANG Shao-qing LU Cheng-ping |
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| Affiliation: | Nanjing Agriculture University, Nanjing 210095, China |
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| Abstract: | A hemolysin gene from six streptcoccus suis type 2 strains isolated in Jiangsu was amplified by PCR using a pair of specific primers.The PCR product from strain HA9801 was sequenced and nucleotide sequence analysis revealed the hemolysin gene from strain HA9801 had a high homology to strains 1933(99%). |
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| Keywords: | streptcoccus suis type 2 suilysin nucleotide sequence |
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