|
|||||
|
|
| Trizol法提取厚皮甜瓜果实RNA条件的筛选 | |
| 引用本文： | 赵继荣,毕阳,刘红霞,高雄杰. Trizol法提取厚皮甜瓜果实RNA条件的筛选[J]. 甘肃农业大学学报, 2009, 44(2) |
| 作者姓名： | 赵继荣 毕阳 刘红霞 高雄杰 |
| 作者单位： | 1. 甘肃农业大学农学院,甘肃,兰州,730070 2. 甘肃农业大学食品科学与工程学院,甘肃,兰州,730070 3. 中国农业科学院作物科学研究所,北京,100081 |
| 摘 要： | 采用改良的Trizol法提取厚皮甜瓜‘银帝’果实中的RNA.利用琼脂糖凝胶电泳、紫外分光光度法和RT-PCR法进行纯度、浓度和RNA完整性检测.结果表明:1 mL Trizol用于提取200 mg甜瓜果实量时,得到的RNA产量最大,氯仿抽提2次时提取到的RNA的A260/A280在1.9~2.0之间,所提取的RNA无蛋白质和DNA污染,室温沉淀和低温沉淀对RNA的产量无明显影响.将提取的RNA反转录后扩增β-actin基因片段,扩增的条带与设计大小一致,验证了该方法提取的RNA可用于RT-PCR. |
| 关 键 词： | Trizol法 甜瓜 RNA 提取 |
Factor screening of RNA extraction from muskmelon fruit by modified Trizol method |
|
| ZHAO Ji-rong,BI Yang,LIU Hong-xia,GAO Xiong-jie. Factor screening of RNA extraction from muskmelon fruit by modified Trizol method[J]. Journal of Gansu Agricultural University, 2009, 44(2) | |
| Authors: | ZHAO Ji-rong BI Yang LIU Hong-xia GAO Xiong-jie |
| Affiliation: | ZHAO Ji-rong1,BI Yang2,LIU Hong-xia3,GAO Xiong-jie2(1.College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China,2.College of Food Science and Engineering,3.Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China) |
| Abstract: | RNA was extracted from muskmelon fruit(Cucumis melo L.cv.Yindi) by modified Trizol method.The purity,concentration and integrity of the extracted RNA were detected by the agarose gel electrophoresis,ultraviolet ray photometer and RT-PCR.The results showed that extraction of RNA reached optimum in 200 mg sample with 1ml Trizol.A260/A280 of RNA arranged 1.9 to 2.0 by chloroform extractive twice.The room temperature and low temperature sediment had no effects on RNA yield.The electrophoresis bands showed clear... |
| Keywords: | RNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |