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A human major histocompatibility complex (MHC) DNA probe recognizes goat genes |
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| Authors: | L L Nesse G Paulsen M Syed G Ruff |
| Affiliation: | 17.The National Veterinary Institute, Oslo, Norway ;27.Department of Microbiology and Immunology, Norwegian College of Veterinary Medicine, Oslo, Institute of Transplantation Immunology, National Hospital, P. O. Box 8156, Dep., N-0033 Oslo 1, Oslo, Norway ;37.Department of Animal Husbandry and Genetics, Norwegian College of Veterinary Medicine, Oslo, Norway ;47.Institute of Animal Breeding, Division of Immunogenetics, Veterinary School of University of Berne, Switzerland |
| Abstract: | A human major histocompatibility complex (MHC) class II (DR-β) probe was hybridized with restriction enzyme digest of genomic DNA from 14 goats. Nine of the animals belonged to one family. Digestion of the DNA with the restriction enzyme Eco RI gave 7 fragments in 13 animals and 6 fragments in the last animal. No other polymorphism could be detected. Bam HI digestion gave from 3 to 6 fragments which displayed a considerable polymorphism. In the family studied, polymorphic fragments were inherited together with serologically defined lymphocyte antigen specificities believed to be coded for by MHC class I genes. |
| Keywords: | lymphocyte antigens restriction fragment length polymorphism RFLP tissue typing tissue antigens |