| 东方铃蟾乳酸脱氢酶多态性研究 |
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| 引用本文： | 曾科文 杨文涛. 东方铃蟾乳酸脱氢酶多态性研究[J]. 野生动物, 1995, 0(4): 31-35 |
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| 作者姓名： | 曾科文 杨文涛 |
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| 作者单位： | 东北林业大学 |
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| 摘 要： | 采用聚丙烯酰胺凝胶电泳分离东方铃蟾不同组织ＬＤＨ同工酶，显示出典型的５条带谱。心肌具有以Ｂ亚基为主的ＬＤＨ同工酶谱，骨骼肌以Ａ亚基为主的ＬＤＨ同工酶谱。用改进后的聚丙烯酰胺凝胶电泳方法进行种群调查，结果表明Ａ位点和Ｂ位点都存在电泳上的变异，各存在二个等位基因。Ａ位点有三个基因型，其频率；ＡＡ＝５３．４７％．Ａａ＝３９．１１％．ａａ＝７．４３％；Ｂ位点只检出二个基因型，即ＢＢ为９９．５０％，Ｂｂ为０．５０％。
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| 关 键 词： | 东方铃蟾（Bombinaorientalis）；乳酸脱氢酶；多态性 |
Studies on Lactate Dehydrogenase Polymorphism in Bombina orientalis |
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| Zeng Kewen,Yang Guangtao,Chen Fengying,Pan Xuefeng. Studies on Lactate Dehydrogenase Polymorphism in Bombina orientalis[J]. , 1995, 0(4): 31-35 |
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| Authors: | Zeng Kewen Yang Guangtao Chen Fengying Pan Xuefeng |
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| Affiliation: | Northeast Forestry University |
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| Abstract: | Polycrylamide gel electrophoresis (PAGE) is used to study LDH isoenzymes in oriental fire bellied toad's different tissues. It shows typical five bands. Ldh-b subunit predominates in heart muscle and Ldh-A subunit in skeletal muscle. Advanced PAGE method is used to investigate toad population. It shows both A and B locus are varied in electrophoresis. Each locus has two alleles. In A locus, three genotypes are found.The frequences are AA 55.47%, Aa 39.11 % and aa 7.43%. In B locus, only two genotypes are found. The frequences are BB 99.5%, Bb 0. 50%. |
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| Keywords: | Bombina orientalis lactate dehydrogenase polymorphism |
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