东方铃蟾乳酸脱氢酶多态性研究 |
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引用本文: | 曾科文,杨文涛.东方铃蟾乳酸脱氢酶多态性研究[J].野生动物,1995(4):31-35. |
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作者姓名: | 曾科文 杨文涛 |
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作者单位: | 东北林业大学 |
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摘 要: | 采用聚丙烯酰胺凝胶电泳分离东方铃蟾不同组织LDH同工酶,显示出典型的5条带谱。心肌具有以B亚基为主的LDH同工酶谱,骨骼肌以A亚基为主的LDH同工酶谱。用改进后的聚丙烯酰胺凝胶电泳方法进行种群调查,结果表明A位点和B位点都存在电泳上的变异,各存在二个等位基因。A位点有三个基因型,其频率;AA=53.47%.Aa=39.11%.aa=7.43%;B位点只检出二个基因型,即BB为99.50%,Bb为0.50%。
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关 键 词: | 东方铃蟾(Bombinaorientalis) 乳酸脱氢酶 多态性 |
Studies on Lactate Dehydrogenase Polymorphism in Bombina orientalis |
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Authors: | Zeng Kewen Yang Guangtao Chen Fengying Pan Xuefeng |
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Institution: | Northeast Forestry University |
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Abstract: | Polycrylamide gel electrophoresis (PAGE) is used to study LDH isoenzymes in oriental fire bellied toad's different tissues. It shows typical five bands. Ldh-b subunit predominates in heart muscle and Ldh-A subunit in skeletal muscle. Advanced PAGE method is used to investigate toad population. It shows both A and B locus are varied in electrophoresis. Each locus has two alleles. In A locus, three genotypes are found.The frequences are AA 55.47%, Aa 39.11 % and aa 7.43%. In B locus, only two genotypes are found. The frequences are BB 99.5%, Bb 0. 50%. |
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Keywords: | Bombina orientalis lactate dehydrogenase polymorphism |
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