| 提高大麦花粉植株再生频率的研究 |
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| 引用本文： | 杜永芹,陈如梅. 提高大麦花粉植株再生频率的研究[J]. 上海交通大学学报(农业科学版), 1993, 0(3) |
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| 作者姓名： | 杜永芹 陈如梅 |
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| 作者单位： | 上海农业科学院作物栽培研究所 上海201106 |
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| 摘 要： | 本文就如何提高大麦花粉植株再生频率进行了研究.结果表明:不同基因型的绿苗再生率存在较大差异,F_1 代明显高于其它世代,平均绿苗率为6.2%.最高达18.3%;用改良 C_(17)培养基作为诱导培养基能有效地提高绿苗率:在诱导培养基中用6%麦芽糖作为碳源有利于愈伤组织的诱导及绿苗的分化;在分化培养基中补加0.5mg/LZT 对绿苗的产生有着明显的促进作用.
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| 关 键 词： | 大麦 花药培养 绿苗频率 |
A STUDY ON IMPROVING THE FREQUENCIES OF GREEN POLLEN PLANTS IN BARLEY ANTHER CULTURE |
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| Du Yongqin Chen Rumei. A STUDY ON IMPROVING THE FREQUENCIES OF GREEN POLLEN PLANTS IN BARLEY ANTHER CULTURE[J]. Journal of Shanghai Jiaotong University (Agricultural Science), 1993, 0(3) |
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| Authors: | Du Yongqin Chen Rumei |
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| Abstract: | The experiments were carried out on the improvement of the frequencies of green pollen plants in barley anther culture The results showed:The differences of green plants rate existed among different genotypes,the green plant frequency of F_1materials was obviously higher than that of others,the average frequency of green plants was 6.2%,with the highest 18.3%. Modified C_(17) medium as in induction medium could effectively enhance frequency of green plants,Addition of 6% maltose as carbon source to induction medium was useful for induc- tion of callus and differentiation of green plants.Addition of 0.5mg/L ZT to regeneration medium had a good effect for the regeneration of green plants. |
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| Keywords: | Barley Anther culture Frequency of green plants |
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