Tissue culture method affects ex vitro growth and development of loblolly pine |
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Authors: | Frampton L. John Amerson Henry V. Leach Gregory N. |
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Affiliation: | (1) Department of Forestry, North Carolina State University, Raleigh, NC 27695-8002, USA;(2) Research and Development, Champion International Corporation, Cantonment, FL 32533, USA |
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Abstract: | Loblolly pine (Pinus taeda L.) propagules initiated adventitiously from cotyledons and via axillary-fascicular micropropagation from epicotyls were compared to seedlings grown from the same open-pollinated seedlot. Greenhouse and early field results indicate that unlike tissue culture propagules of cotyledon-origin, propagules originating via axillary-fascicular micropropagation from epicotyls do not display early reduced growth relative to seedlings. Further, axillary-fascicular micropropagules derived from cotyledon-origin adventitious shoots displayed early reduced growth. Early reduced growth and other differences between tissue culture propagules and seedlings previously observed in field plantings appear to be inherent to the cotyledon system of propagation and do not necessarily occur when other tissue culture procedures are used for propagation. |
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Keywords: | field performance growth pattern juvenility maturation micropropagation planting stock Pinus taeda L. |
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