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炭疽菌附着胞形成的影响因素及形态特征比较
引用本文:陈玉森,叶乃兴,许文耀,刘威,魏日凤,刘伟.炭疽菌附着胞形成的影响因素及形态特征比较[J].福建农林大学学报(自然科学版),2013,42(2).
作者姓名:陈玉森  叶乃兴  许文耀  刘威  魏日凤  刘伟
作者单位:1. 福建农林大学植物保护学院
2. 福建农林大学茶叶科技与经济研究所;福建农林大学园艺学院,福建福州350002
3. 福建农林大学园艺学院,福建福州,350002
4. 福建农林大学茶叶科技与经济研究所;宁德师范学院,福建宁德352100
基金项目:国家自然科学基金项目,公益性行业(农业)科研专项,福建农林大学园艺植物种质与高优生产技术创新团队项目,福建省高校新世纪优秀人才资助项目,福建省农科教项目,福建省企业技术创新专项,福建省农业五新工程项目
摘    要:研究了炭疽菌属的孢子萌发率、附着胞形成率及形态等受孢子龄、叶汁种类和孢子悬液浓度影响的情况.结果表明,5 d菌龄的孢子以水配成3.4×105个·mL-1左右的孢子悬液,在25℃光照下培养12 h,附着胞的形态特征稳定,较适宜孢子萌发与附着孢形态的观察.对附着胞形态观察发现,11种炭疽菌产生的附着胞特征均存在一定的差异;胶孢炭疽菌的12个菌株中,除寄生于雷公藤的炭疽菌外,其余11个菌株的附着孢形态差异不明显.

关 键 词:炭疽菌  附着胞  形态特征  影响因素

Factors affecting appressorium formation and its morphological comparison in Colletotrichum
CHEN Yu-sen , YE Nai-xing , XU Wen-yao , LIU Wei , WEI Ri-feng , LIU Wei.Factors affecting appressorium formation and its morphological comparison in Colletotrichum[J].Journal of Fujian Agricultural and Forestry University,2013,42(2).
Authors:CHEN Yu-sen  YE Nai-xing  XU Wen-yao  LIU Wei  WEI Ri-feng  LIU Wei
Abstract:The effects of spore ages,spore concentrations and different leaf liquors on sporulation rate,appressorium formation rate and morphology in Colletotrichum were investigated.The results showed that when 5 days old spores diluted 3.4×105 individuals·mL-1 spore concentration with water were incubated for 12 h under 25 ℃ with light,appressoria were formed with stable morphology.The above conditions were considered suitable for sporulation and appressorium observation.On one hand,the appressorium morphology was found slightly different in the 11 different Colletotrichum species.On the other hand,the differences of appressorium morphology were similar in the 12 strains of C.gloeosporioides,except the one from Tripterygium wilfordii.
Keywords:Colletotrichum  appressorium  microscopic morphology  effect factor
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