Development of a specific radioimmunoassay for vitamin B12 and its application in the diagnosis of cobalt deficiency in sheep |
| |
Authors: | D. G. Kennedy F. P. M. O'Harte W. J. Blanchflower D. A. Rice |
| |
Affiliation: | (1) Veterinary Research Laboratories, Stoney Road, Stormont, BT4 3SD Belfast, UK |
| |
Abstract: | A radioimmunoassay (RIA) for vitamin B12 is described. Antisera were raised in rabbits using a conjugate between 5-O-succinyl cyanocobalamin and chicken serum albumin. The antibody is largely specific for the (upper) face of the vitamin B12 molecule. The RIA can detect vitamin B12 down to concentrations of 37 pmol/L and correlated well (r=0.980, p<0.001) with a commercial radioassay. The use of this RIA, together with an assay for plasma methylmalonic acid, in the diagnosis of cobalt/vitamin B12 deficiency in sheep is described. |
| |
Keywords: | cobalt diagnosis radioimmunoassay sheep vitamin B12 |
本文献已被 SpringerLink 等数据库收录! |