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Proposal of screening method for intestinal mucus adhesive lactobacilli using the enzymatic activity of glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) |
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| Authors: | Hideki KINOSHITA Shun IMOTO Yoshihito SUDA Mitsuharu ISHIDA Masamichi WATANABE Yasushi KAWAI Haruki KITAZAWA Koh MIURA Akira HORII Tadao SAITO |
| Affiliation: | 1. Department of Food Management, School of Food, Agricultural and Environmental Sciences, Miyagi University;2. Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University;3. Division of Biological Regulation and Oncology, Department of Surgery;4. Division of Molecular Pathology, Department of Pathology, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan |
| Abstract: | Adhesion tests are complex, time‐consuming and expensive, while the most important criterion for a probiotic lactobacilli is the ability to adhere to the human intestine. Thirty lactobacilli isolates from human intestinal tissues were measured for cell surface glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) activity using a microtiter plate screening method. GAPDH activities were detected in 21 out of 30 samples from 12 h cultures and in all samples from 18 h cultures. This suggests GAPDH is universally expressed on the bacterial cell surfaces from many lactobacilli. A statistically significant positive correlation was shown between GAPDH activity and adhesion using the BIACORE adhesion assay (P < 0.01). The new screening method using GAPDH enzymatic activity without an adhesion test may be possible due to the significant positive correlation of GAPDH activity with adhesion of lactobacilli derived from the human intestine. |
| Keywords: | adhesion enzymatic activity glyceraldehyde‐3‐phosphate dehydrogenase lactobacilli probiotics |