首页 | 本学科首页   官方微博 | 高级检索  
     检索      


Time course of changes in antioxidant activity of milk from dairy cows fed a trehalose‐supplemented diet
Authors:Naoto AOKI  Kan SATO  Shuhei KANDA  Kazuhisa MUKAI  Yoshiaki OBARA  Hisao ITABASHI
Institution:1. Department of Biological Production, Tokyo University of Agriculture and Technology, Fuchu;2. Hayashibara Biochemical Laboratories Inc, Okayama;3. Mito Experimental Farm, Meiji Feed Co. Ltd, Ibaraki, Japan;4. Faculty of Applied Life Science, Nippon Veterinary and Life Science University, Musashino, Tokyo
Abstract:This study was to investigate the time course of changes to the antioxidant activity of milk from cows fed a trehalose‐supplemented diet, and to determine possible underlying mechanisms for observed changes. Six Holstein cows were used, and subjected to two experimental feeding periods consisting of a 1% trehalose‐supplemented diet for 10 days, followed by a basal diet only (no trehalose) for 10 days. 1,1‐Diphenyl‐2‐picrylhydrazyl (DPPH) radical scavenging activities in milk were gradually increased during the trehalose supplementation period and were highest at the end of the second period. However, trehalose was not detected in the milk and plasma of dairy cows fed a diet supplemented with trehalose for 10 days, indicating that the increased antioxidant activity in the milk of trehalose‐fed cows is not due to the direct transfer of trehalose to the milk. Plasma DPPH activities exhibited a similar time course to that seen for milk. Relative superoxide dismutase (SOD) activities in the rumen were higher 3 days after the end of trehalose supplementation than at any other time during the experimental periods. These results suggested that the improved antioxidant activity in milk and plasma of cows fed a trehalose‐supplemented diet was due to improved ruminal relative SOD activity.
Keywords:antioxidant activity  dairy cow  rumen  trehalose
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号