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D-氨基酸抑制植物生长的现象
引用本文:赵大华,杨靖,李友勇. D-氨基酸抑制植物生长的现象[J]. 华北农学报, 2007, 22(2): 60-63
作者姓名:赵大华  杨靖  李友勇
作者单位:1. 河南科技学院,生物技术系,河南,新乡,453003;华南农业大学,生命科学学院,广东,广州,510642
2. 河南科技学院,生物技术系,河南,新乡,453003
基金项目:河南省自然科学基金 , 河南省教育厅自然科学基金
摘    要:丙氨酸、丝氨酸、缬氨酸和蛋氨酸4种氨基酸的L-和D-异构体对小麦胚试管植株生长影响的试验结果表明,丙氨酸和丝氨酸的D-异构体在3 mmol/L以上浓度强烈抑制试管植株生长,L-异构体不抑制或轻的多,这是少见的D-氨基酸抑制植物生长的现象。把在丙氨酸、丝氨酸处理中生长4 d的已经受到抑制的植株再转入无氨基酸培养基后,植株可恢复生长,这显示D-丙氨酸和D-丝氨酸的抑制作用可能有反馈机制存在。D-氨基酸的这些作用在筛选植物生长延缓剂、离体植物遗传标记等方面有重要价值。

关 键 词:D-氨基酸  丙氨酸  丝氨酸  反馈抑制  离体小麦胚
文章编号:1000-7091(2007)02-0060-04
修稿时间:2006-07-10

The Inhibition of D-amino Acid on Plant Growth
ZHAO Da-hua,YANG Jing,LI You-yong. The Inhibition of D-amino Acid on Plant Growth[J]. Acta Agriculturae Boreali-Sinica, 2007, 22(2): 60-63
Authors:ZHAO Da-hua  YANG Jing  LI You-yong
Affiliation:1. Department of Biotechnology, Henan Institute of Science and Technology,Xinxiang 453003,China; 2. College of Life Science,South China Agricultural University, Guangzhou 510642,China
Abstract:The effect of L-and D-isomer of four kinds of amino acids(Alanine,Serine,Methionine and Valine) with different concentration on seedlings of wheat embryos in vitro was studied.The results showed that D-isomers of Alanine and Serine could strongly inhibit seedling growth when their concentration reached to 3 mmol/L,but their L-isomers had much less or no effect.It belongs to a rare phenomenon that the D-amino acids inhibit plant growth.When the seedlings enduring fourday inhibition by D-alanine and D-serine were transferred into the medium containing no amino acid,their growth recovered again.This suggested that there might exist a feedback mechanism for the D-amino acid inhibiting effect,which was of great importance to screen plant growth retardants and plant genetic markers in vitro.
Keywords:D-amino acid  Alanine  Serine  Feedback inhibition  Wheat embryos in vitro
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