| 巴西橡胶芽接树砧穗互作的蛋白质组初步研究 |
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| 引用本文： | 丁璇,袁坤,杨礼富,曹建华,徐志娟,何哲,林位夫. 巴西橡胶芽接树砧穗互作的蛋白质组初步研究[J]. 热带作物学报, 2010, 31(9): 1514-1518. DOI: 10.3969/j.issn.1000-2561.2010.09.015 |
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| 作者姓名： | 丁璇 袁坤 杨礼富 曹建华 徐志娟 何哲 林位夫 |
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| 作者单位： | 1中国热带农业科学院橡胶研究所 2农业部橡胶树生物学重点开放实验室 3海南大学农学院;1中国热带农业科学院橡胶研究所 2农业部橡胶树生物学重点开放实验室;1中国热带农业科学院橡胶研究所 2农业部橡胶树生物学重点开放实验室;1中国热带农业科学院橡胶研究所 2农业部橡胶树生物学重点开放实验室;1中国热带农业科学院橡胶研究所 3海南大学农学院;1中国热带农业科学院橡胶研究所 3海南大学农学院;1中国热带农业科学院橡胶研究所 2农业部橡胶树生物学重点开放实验室 |
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| 基金项目： | 农业部公益性行业科研专项,农业部科研院所基本业务费项目,天然橡胶产业技术体系,海南省自然科学基金项目 |
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| 摘 要： | 运用蛋白质组学研究技术,寻找砧木和接穗互作相关蛋白。以巴西橡胶树3种芽接组合植株的砧木与接穗结合部附近处的胶乳为试验材料,经双向凝胶电泳(2-DE)分离获得胶乳中存在的差异表达蛋白,采用质谱技术鉴定出32个差异表达蛋白,这些蛋白主要参与转录、翻译、光合作用、细胞分裂、蛋白质合成和胶乳合成等生物学过程。
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| 关 键 词： | 橡胶树 砧木 接穗 相互作用 蛋白质组 双向电泳 质谱 |
The Preliminary Proteome Study on the Interaction between Rootstock and Scion of Budgrafted Rubber Tree |
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| Ding Xuan,Yuan Kun,Yang Lifu,Cao Jianhu,Xuzhijuan,Hezhe and Lin Weifu. The Preliminary Proteome Study on the Interaction between Rootstock and Scion of Budgrafted Rubber Tree[J]. Chinese Journal of Tropical Crops, 2010, 31(9): 1514-1518. DOI: 10.3969/j.issn.1000-2561.2010.09.015 |
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| Authors: | Ding Xuan Yuan Kun Yang Lifu Cao Jianhu Xuzhijuan Hezhe Lin Weifu |
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| Affiliation: | 1 Rubber Research Institute, Chinese Academy of Tropical Agriculture Sciences 2 Key Laboratory of Rubber Tree Biology of Ministry of Agriculture 3 College of Agronomy of Hainan University;1 Rubber Research Institute, Chinese Academy of Tropical Agriculture Sciences 2 Key Laboratory of Rubber Tree Biology of Ministry of Agriculture;1 Rubber Research Institute, Chinese Academy of Tropical Agriculture Sciences 2 Key Laboratory of Rubber Tree Biology of Ministry of Agriculture;1 Rubber Research Institute, Chinese Academy of Tropical Agriculture Sciences 2 Key Laboratory of Rubber Tree Biology of Ministry of Agriculture;1 Rubber Research Institute, Chinese Academy of Tropical Agriculture Sciences 3 College of Agronomy of Hainan University;1 Rubber Research Institute, Chinese Academy of Tropical Agriculture Sciences 3 College of Agronomy of Hainan University;1 Rubber Research Institute, Chinese Academy of Tropical Agriculture Sciences 2 Key Laboratory of Rubber Tree Biology of Ministry of Agriculture |
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| Abstract: | The proteomics research technique was applied to find the proteins related to the interaction between rootstock and scion. Latex colleced from the rootstock and scion parts of 3 budgrafted rubber trees as the experimental materials, differentially expressed proteins were obtained by 2-DE. Using MS technique, 32 differentially expressed proteins were identified. These proteins were mainly involved in transcription, translation, photosynthesis, cell division, protein synthesis and latex synthesis. |
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| Keywords: | Rubber tree(Hevea brasiliensis) Rootstock scion Interaction Proteome 2-DE MS. |
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