| 人参组织培养的多因子正交试验研究 |
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| 引用本文： | 王建华,王义,孙国伟,张美萍. 人参组织培养的多因子正交试验研究[J]. 吉林农业大学学报, 2006, 28(6): 648-651 |
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| 作者姓名： | 王建华 王义 孙国伟 张美萍 |
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| 作者单位： | 吉林农业大学生物技术学院,长春,130118;长春市朝阳区富锋镇农业站,长春,130103 |
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| 基金项目： | 吉林省科技发展计划资助项目(20051113) |
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| 摘 要： | 采用L9(34)正交设计分析比较了2.4-D,BA,NAA,KT 4种激素浓度及其组合对人参组织培养的影响。经直观分析、方差分析和多重比较进行综合评价,确定人参愈伤组织诱导的最优培养基为MS 2.4-D3 mg/L BA 0.5 mg/L NAA 0.5 mg/L KT 0.5 mg/L,人参愈伤组织生长的最优培养基为MS 2.4-D 3 mg/L KT 1 mg/L,人参不定芽发生的最优培养基为MS BA 1 mg/L。
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| 关 键 词： | 人参 正交设计 组织培养 |
| 文章编号： | 1000-5684（2006）06-0648-04 |
| 收稿时间： | 2005-11-18 |
| 修稿时间： | 2005-11-182006-04-16 |
Study on Tissue Culture of Panax ginseng by Applying Orthogonal Design |
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| WANG Jian-hua,WANG Yi,SUN Guo-wei,ZHANG Mei-ping. Study on Tissue Culture of Panax ginseng by Applying Orthogonal Design[J]. Journal of Jilin Agricultural University, 2006, 28(6): 648-651 |
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| Authors: | WANG Jian-hua WANG Yi SUN Guo-wei ZHANG Mei-ping |
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| Affiliation: | 1. College of Biotechnology , Jilin Agricultural University, Changchun 130118, China ; 2.Agrotechnical Experiment Farm, Fufeng Town, Chaoyang District, Changchun 130103, China |
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| Abstract: | The effects of 2.4-D,BA,NAA and KT in different combinations and different concentrations on callus induction,growth and shoot propagation of Panax ginsengwere studied by using orthogonal design L_9(3~4).The data subjected to visual analysis,variance analysis and multiple comparison.The results indicated the optimum media were: MS 2.4-D 3 mg/L BA 0.5 mg/L NAA 0.5 mg/L KT(0.5 mg/L) for callus induction,MS 2.4-D 3 mg/L KT 1 mg/L for callus growth and MS BA(1 mg/L) for shoot propagation. |
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| Keywords: | Panax ginseng orthogonal design tissue culture |
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