Canine C-reactive protein (CRP) does not share common antigenicity with human CRP |
| |
Authors: | S. Yamamoto S. Miyaji N. Abe K. Otabe E. Furukawa M. Naiki |
| |
Affiliation: | (1) Department of Immunology, Faculty of Environmental Health, Azabu University, Fuchinobe, Sagamihara, 229 Kanagawa, Japan;(2) Sanwa Kagaku Kenkyusyo Co. Ltd., Higashiku, Nagoya, 461 Aichi, Japan;(3) Department of Veterinary Science, The National Institute of Health, Shinjuku, 162 Tokyo, Japan |
| |
Abstract: | Differences in antigenicity between human and canine C-reactive proteins were investigated by Western blotting analysis. It was confirmed that several commercial anti-human CRP sera reacted with canine CRP. However, 34 anti-canine CRP sera prepared by immunization of rabbits and goats with canine CRP all reacted with canine CRP but not with human CRP in either immunoelectrophoresis or Western blotting.Immunization with human CRP produced a cross-reacting antibody that reacted with canine CRP. Conversely, immunization with canine CRP did not produce a cross-reacting antibody that reacted with human CRP. These findings may be interpreted as showing that, while canine and human CRPs do not share common antigenicity, they do contain structurally similar antigenic determinants.Abbreviations CRP C-reactive protein - PBS phosphate-buffered saline - SDS-PAGE sodium dodecyl sulphate-polyacrylamide gel electrophoresis |
| |
Keywords: | antigen C-reactive protein dog epitopes immunoelectrophoresis human serology Western blot |
本文献已被 SpringerLink 等数据库收录! |
|