马铃薯蛋白酶抑制剂-Ⅱ基因在转基因水稻中的遗传及表达

 以水稻光敏不育系浙农大 115 (ZAU11S)和 3个转基因品系杂交 ,研究了其F2 群体中bar、pinII基因的遗传及其表达行为。通过涂抹除草剂Basta,进行PCR分析和测定胰蛋白酶被抑制活性 ,发现F2 代bar和pinII基因按孟德尔方式遗传并紧密连锁 ,但在部分植株中bar基因表达不充分 ;作为创伤诱导的pinII基因具有明显的时空表达特性 ,其诱导信号传导不仅向上 ,也向下传导 ;不同水稻转基因系中pinII基因的诱导表达不完全一致。

Inheritance and Expression of Potato Proteinase Inhibitor Gene Ⅱ(pinll) in Transgenic Rice

The inheritance and expression of bar gene and pinII gene were studied in three transgenic rice lines and their F 2 hybrid populations which were created through hybridization with a PGMS line, ZAU11S. By Basta painting, PCR analysis and determining the inhibitory trypsin activity, the results show that bar gene and pinII gene in F 2 population fit the simple Mendel's low of inheritance and close linkage, but a few plants in F 2 have not sufficiently expressed. The wound inducible pinII gene has an expression regulated spatially and temporally, and the signal transduction pathway is not only upward, but also downward. The inducible expression of pinII in different rice transgenic lines is not completely coincident.