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青海湖裸鲤乳酸脱氢酶的研究
引用本文:李太平,赫广春,赵凯,吴华,李军祥. 青海湖裸鲤乳酸脱氢酶的研究[J]. 黑龙江畜牧兽医, 2001, 0(10): 7-8
作者姓名:李太平  赫广春  赵凯  吴华  李军祥
作者单位:[1]青海大学农牧学院动物科学系,青海西宁810003 [2]青海省水产研究所,青海西宁810012
基金项目:国家自然科学基金 (项目编号 :3986 0 0 6 0 ),教育部《高等学校骨干教师资助计划》项目资助
摘    要:采用聚丙烯酰胺凝胶电泳法对青海湖裸鲤的心脏,肾脏,肝脏,鳃, 肉和眼睛6种组织的乳酸脱氢酶同工酶进行了研究,同时与草鱼的相应组织做了比较。发现青海湖裸鲤有17条亚带,并用热变形实验初步确定这些亚带分属5种LDH同工酶,草鱼有11条亚带,并在其肾脏发现有X区带。

关 键 词:青海湖裸鲤 乳酸脱氢酶 同工酶 聚丙烯酰胺凝胶电泳
文章编号:1004-7034(2001)10-0007-02
修稿时间:2001-06-14

Investigation on Isozyme of Lacate Dehydrogenase in Naked Carps in Qinghai-lake
LI Tai-ping,LI Jun-xiang,ZHAO Kai,WU Hua. Investigation on Isozyme of Lacate Dehydrogenase in Naked Carps in Qinghai-lake[J]. Heilongjiang Animal Science And veterinary Medicine, 2001, 0(10): 7-8
Authors:LI Tai-ping  LI Jun-xiang  ZHAO Kai  WU Hua
Abstract:Six tissues(heart,kindey,liver,gill,muscle and eyeball)of naked carps (G.przewalskii)were used to investigate lacatedehydrogenase(LDH) isozymes by means of polyacrylamide gelelectrophoresis.17 subbands were found and shown initially to belong tofive LDH isozymes by means hot denaturation method.The isozyme-spectraof lacate dehydrogenase were compared in the corresponding tissuesbetween he naked carps and the grass carps.11 subbands were foud intissues of grass carp,including a X band.
Keywords:qinghai-lake naked carps  lacate dehydrogenase  isozyme
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